摘要
目的制备多西他赛纳米脂质载体,并考察其剂型性质及体外释药行为。方法采用薄膜超声法制备多西他赛纳米脂质载体,使用透射电镜观察粒子外观形态,光子相关光谱法测定其粒径和分布,微柱离心法测包封率,透析法测体外释药特性。结果在优化条件下制备的纳米级粒子粒径为(124±16)nm,Zeta电位为-19.57 mV,包封率为95.8%。体外释放符合Weibull释放模型。结论制备的多西他赛纳米脂质载体粒径小,药物包封率高,并可实现药物控释。
Objective To prepare docetaxel nanostructured lipid carriers （docetaxel-NLC） and investigate their pharmaceutical properties and drug releasing behavior in vitro. Methods Docetaxel-NLC was formulated by film-dispersion and ultrasonication method. Transmission electron microscopy （TEM） was employed to study the shape. Photon correlation spectroscopy （PCS） was used to study the diameter. The entrapment efficiency was determined by mini-column centrifugation. The in vitro drug releasing behavior was studied by dialysis. Results The mean diameter of NLC was （124 ± 16） nm with Zeta potential of -19.57 mV, and the mean entrapment efficiency was 95.8 %. The in vitro drug release was fitted well with Weibull equation. Conclusions Docetaxel-NLC with small particle sizes can improve drug loading capacity and its controlled releasing properties.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2008年第3期174-177,共4页
Journal of Shenyang Pharmaceutical University
基金
辽宁省科技厅科技项目(2007402071)
关键词
多西他赛
纳米脂质载体
包封率
体外释放
docetaxel
nanostructured lipid carriers
entrapment efficiency
in vitro drug release
作者简介
杨平（1980-），女（汉族），黑龙江大庆人，硕士研究生，E—mail：yangping2006@gmail．com；
何仲贵（1965-）男（汉族），宁夏盐池人，教授，主要从事药物新剂型及新技术的研究，Tel．024-23986321。E-mail：hezhonggui@gmail．com。
