摘要
甘油为微生物可利用的理想碳源,从自然界筛选出21株以甘油为唯一碳源产二羟丙酮(DHA)的菌株,经初步发酵测定发酵液中DHA含量,其中菌株6-8DHA产量最高达6.4g/L。对其进行常规生理生化鉴定实验,并结合16SrDNA基因分析,比对结果表明,菌株6-8与Acinetobacter sp.相似性最高,达99.7%,在细菌分类学上属于假单胞菌目莫拉菌科不动杆菌属。将其命名为Acinetobacter sp.6-8。
More than 20 strains capable of producing dihydroxyacetone from glycerol were isolated from 4 different natural environment samples by using two detection methods, The strain 6-8 which could grow on medium containing glycerol as sole carbon source had a higher converting capability. Under a better culture, the highest DHA production of the strain 6-8 reached 6.4 g/L. In addition to general morphological and biochemical characteristics, the strain 6-8 was identified by 16S rDNA sequence and systematic analysis. The results showed that 16S rDNA sequence of the strain 6-8 had similarity of 99.7% with Acinetobacter sp. suggesting that the strain 6-8 is one of subspecies of Acinetobacter sp.
出处
《微生物学通报》
CAS
CSCD
北大核心
2008年第3期336-340,共5页
Microbiology China
基金
国家自然科学基金(No.20676053)
国家"863计划"(No.2006AA020103)
江苏省青年科技创新人才(学术带头人)基金(No.BK2006504)
长江学者和创新团队发展计划资助(No.IRT0532)
关键词
甘油
二羟基丙酮
分离筛选
鉴定
Glycerol, Dihydroxyacetone, Screening, Identification
作者简介
Tel：0510-85918109；E-mail：raozm@yahoo.com.cn
E-mail：fanghuiying@126.com
