摘要
在模拟阳性泡菜样品中寄生虫卵分离的基础上,本研究通过对单蛔虫卵分别进行加热、液氮/37℃反复冻融、PCR缓冲液冻融、裂解液消化等四种前处理后进行荧光PCR检测,表明将虫卵进行液氮/37℃反复冻融15次后用于荧光PCR检测的效果确实而稳定。所建立的蛔虫卵荧光PCR检测方法特异性好,与鞭虫、毛圆线虫等动物寄生虫线虫不存在交叉反应,可以很好的区分蛔虫与其它动植物线虫卵。敏感性可以满足单个蛔虫卵的检测需要。本方法的建立为泡菜等植物性产品中携带的疑似蛔虫卵的鉴定提供了科学依据。
To identify the Ascaris egg- like materials from pickles, after the isolation of parasitic eggs from pickles, a real-time PCR assay for identifying Ascaris egg was set up and optimized. By comparison, the repeating treatment of the single egg in liquid nitrogen freezing with 37 ℃ thawing for 15 cycles is the best process other than 95 ℃ heating, and -86 ℃ freezing or 37 ℃thawing in PCR buffer, or lysis with proteinase K digestion in the pre-treatment of the Ascaris egg. The subsequently developed real-time PCR detection is of much specificity and sensitivity for the Ascairs egg identification and can be used satisfactorily in the inspection service.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第11期345-349,共5页
Food Science
关键词
泡菜
蛔虫卵
鉴定
荧光PCR
食品
寄生虫
pickles
Ascaris egg
identification
real-time PCR（,polymerase chain reaction）
food
parasite
作者简介
吴绍强（1970-），男，副研究员，博士，主要从事动物检疫及人兽共患病研究。
通讯作者
