摘要
为同时测定食品中的副溶血性弧菌和金黄色葡萄球菌,建立了基于TaqMan探针的双重Real timePCR方法。针对副溶血性弧菌的gyrB基因序列和金黄色葡萄球菌coa基因序列分别设计引物和TaqMan探针,建立双重Real timePCR检测体系,制作校正曲线,同步定量检测副溶血性弧菌和金黄色葡萄球菌。建立的双重Real timePCR方法对2种细菌菌液的检测敏感度均低于10CFU PCR反应体系,相关系数均为1.00,整个试验可在2h内完成。建立的方法可用于食品中副溶血性弧菌和金黄色葡萄球菌的快速、同步、定量检测。
A duplex Real-time PCR assay for quantitative detection of V. parahaemolyticus and Staphylococcus aureus was presented. The primers and probe were designed according to the gyrB gene sequence of V. parahaemolyticus strains and the coa gene sequence of S. aureus strains, respectively. The sensitivity of the assay was less than 10 CFU per PCR Mixture, and the correlation rate was 1.0 （ γ^2 = 1.0）. The assay could be completed within 2 h. The assay presented here can be used as a rapid screening tool for simultaneous and quantitative detection of V. parahaemolyticus and S. aureus in foods without the need of preisolation and characterization of the bacteria by traditional microbiological methods.
出处
《中国食品卫生杂志》
2006年第3期205-209,共5页
Chinese Journal of Food Hygiene
作者简介
蒋鲁岩 男 高级工程师.
通讯作者：陈溥言 男 教授 .
