摘要
研究了不同培养基、采集时间、贮藏温度和湿度等因素对百日草花粉离体培养萌发效果的影响。结果表明:花粉萌发较适宜的培养基为ME3+16%PEG4000+12%蔗糖;上午10:00采集花粉萌发率要显著地高于下午3:00采集的;在4℃湿润条件下贮藏18 h,J6花粉生活力下降较小,而在室温干燥条件下几乎失去活力;相对湿度在66%以下时极不利于花粉保存;11个百日草自交系花粉离体萌发率差异显著。
The factors on different culture medium, collecting time, storage temperature and relative humidity affecting pollen germination of Zinnia elegans were studied. The results are as follows：The optimum germination medium in vitro pollen germination of Zinnia elegans is a basic medium ME3 supplemented with 16% PEG4000 and 12% sucrose. The pollens germination rate which pollens is collected at 10：00 am is signifieantly different to that at 3.00 pm. The pollen germination rate of J6 reduced more little at 4 ℃ and moist for 18 h,but it almost lost all at room temperature and dry. Pollen stored very bad below 66% of relative humidity. Pollens germination of 11 Zinnia elegans cultivars cultured on the medium are significantly different.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2007年第5期693-696,共4页
Journal of Huazhong Agricultural University
基金
国家"948"项目(2003-Z36)资助
关键词
百日草
自交系
离体花粉
萌发
花粉生活力
Zinnia elegans Jacq.
inbred lines
in vitro pollen
germination
pollen vitality
作者简介
叶要妹，女，1965年生，副教授．华中农业大学园艺林学学院在职博士研究生，武汉430070
通讯作者．E-mail：mzbao@mail．hzau．edu．cn
