摘要
建立了一种同时测定水产品(鳗鱼、鮰鱼、龙虾)中13种磺胺类药物残留的高效液相色谱法。样品经乙腈提取,正己烷液-液分配,阳离子固相萃取小柱净化后,采用高效液相色谱分离,紫外检测器检测,外标法定量。13种磺胺类药物在0.1～5.0μg/ml范围内线性关系良好,相关系数R2为0.9997～0.9999,加标回收率为62%～97%,相对标准偏差为2.01%～10.46%。定量测定低限磺胺二甲嘧啶、磺胺甲氧嘧啶、磺胺-6-甲氧嘧啶、磺胺氯哒嗪、磺胺甲基异恶唑、磺胺二甲异恶唑、磺胺间二甲氧嘧啶、磺胺喹恶啉为0.02mg/kg,磺胺嘧啶、磺胺吡啶、磺胺噻唑、磺胺甲基嘧啶、磺胺甲噻二唑为0.05mg/kg。
A high performance liquid chromatographic method has been developed for the simultaneous determination of thirteen sulfonamides residues in aquatic products（eels, longsnout catfish and langouste）. The samples were extracted with acetonitrile, then cleaned up by n-hexane liquid-liquid extraction and solid phase extraction. The residues were determined by high perfor- mance liquid chromatography with ultraviolet detection and quantified by external standard method. The detection limits were 0.02mg/kg for sulfamethazine （SM2）, sulfameter （SMT）, sulfamonomethoxine（SMM）, sulfachloropyridazine（SCP）, sulfamethoxazole（SM-Z）, sulfisoxazole（SIX）, sulfadimethoxine（SDM） and sulfaquinoxaline（SQ）, and 0.05 mg/kg for sulfadiazine（SD）, sulfapyridine（SPD）, sulfathiazole（ST）, sulfamerazine（SM1） and sulfamethizole（STZ）. The linear ranges were 0.1～5.0μg/ml. The average recoveries in spiked sample were 62%～97%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第10期448-451,共4页
Food Science
作者简介
陈振桂（1983-），男，硕士研究生，研究方向为食品安全。
通讯作者
