摘要
目的研究猪苓多糖长循环脂质体的处方和制备工艺。方法采用逆相蒸发法制备脂质体。以紫外分光光度-Sephadex法测定脂质体中猪苓多糖的包封率和载药量,采用单因素和正交试验法优化猪苓多糖长循环脂质体的处方和制备工艺。结果优化后的脂质体平均包封率为98.24%,平均载药量为27.20%。结论脂质体制备工艺合理,紫外分光光度-Sephadex法测定猪苓多糖长循环脂质体包封率准确性高,重现性好,简便易行。
Objective To study the processing techniques for prescription and preparation of Polyporus umbellatus polysaccharides long circulating liposomes （PUPS LCLs）. Methods The liposomes were prepared by the reverse evaporating method. The main influencing factors were identified by single factor analysis, then the techniques of the prescription and preparation of liposomes were optimized. The entrapment efficiency and the carrying amount were determined by UV-Sephadex method. Results After optimized, the average entrapment efficiency of liposomes was 98.24% and the average carrying amount was 27.20%. Conclusion Preparation method of PUPS LCLs is available. UV-Sephadex method is simple and suitable for quality control of PUPS LCLs with desirable reproducibility and the results are reliable.
出处
《中草药》
CAS
CSCD
北大核心
2007年第10期1484-1487,共4页
Chinese Traditional and Herbal Drugs
关键词
猪苓多糖
长循环脂质体
逆相蒸发法
正交试验法
制备
Polyporus umbellatus polysaccharides （PUPS）
long circulating liposomes （LCLs）
reverse evaporating method
orthogonal test
preparation
作者简介
汪继红（1976-），女，湖北省应城市人，讲师，硕士，研究方向为药物新剂型的研制与开发。Tel：（027）83657549 E-mail：Wj200510@126．com
