摘要
目的对基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)检测与鉴定常见食品真菌进行探索。方法对MALDI-TOF-MS检测食品真菌所用基质、基质与样品比例、上样方法等进行筛选,获得优化的实验条件,并对方法的重现性和实际应用进行研究。结果MALDI-TOF-MS分析不同属真菌所需的最适基质各异,红曲霉属与镰孢菌属最适基质均是MBT;曲霉属、青霉属与酵母菌的最适基质均是IAA;上样的最适基质/样品比例为1∶1;混合液滴干燥法为最佳上样方法。结论MALDI-TOF-MS直接分析真菌的方法稳定、重现性好。
Objective To examine and identify fungi with important significance in food safety by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS）. Method Sample preparation, screening of appropriate matrix for specific fungal analysis, suitable ratio of matrix to sample, methods for loading samples on mass spectrometer sample tray and repeatability were investigated to optimize the parameters for fungal detection and identification by MALDI-TOF-MS. Results The results showed that different genus of fungi owned their proper matrix in sample preparation ： MBT for both Monascus and Fusaruim species, IAA for Aspergillus and PeniciUium species as well as yeast, respectively, The optimum ratio of matrix to sample was 1 ： 1 （v/v）, Dried droplet is the best choice for applying sample on sample tray of the mass spectrometer. Conclusion MALDI-TOF-MS is of stability and repeatability characteristics in detecting and identifying fungus.
出处
《中国食品卫生杂志》
2007年第5期385-393,共9页
Chinese Journal of Food Hygiene
基金
国家自然基金资助课题(30471456)
国家"十一五"科技支撑计划资助课题(2006BAK02A27)
关键词
光谱法
质量
基质辅助激光解吸电离
真菌
化学
分析
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Fungi
Chemistry, Analytical
作者简介
李凤琴 女 研究员
