摘要
采用双向电泳技术分析了大豆种子萌发时期蛋白质的差异表达。研究发现,在考染胶上,通过PDQuest分析,在成熟干种子和萌发种子的2-DE图谱上分别获得341和376个蛋白斑点,两个时期的2-DE图谱非常相近,说明绝大部分大豆种子贮藏蛋白在萌发期尚未开始降解。有21个蛋白点在萌发的大豆种子中表达量上调,9个表达量下调。对这些蛋白的进一步鉴定将有助于深入理解大豆种子萌发过程的生理及分子机制。
Two-dimensional gel electrophoresis （2-DE） was utilized to analyze proteins with differential expressions during soybean seed germination. It was found that 341 and 376 protein spots were present in dry mature seeds and germinated seeds in 2-DE maps, stained by coomassie brilliant blue and analyzed by PDQuest image software. The 2-DE map of germinated seed was very similar to that of dry mature seed, suggesting that most of soybean seed storage proteins weren＇t mobilized during seed germination. And 21 up-regulated proteins and 9 down-regulated proteins were found during seed germination. The further identification for these protein spots would help to understand the physiological and molecular mechanisms of soybean seed germination.
出处
《大豆科学》
CAS
CSCD
北大核心
2006年第4期355-359,共5页
Soybean Science
基金
教育部跨世纪优秀人才基金和农业部"948"项目
关键词
大豆
双向电泳(2-DE)
萌发
Soybean
Two-dimensional gel electrophoresis （2-DE）
Germination
作者简介
徐晓燕（1982-），硕士，研究方向；植物基因工程。
通讯作者：喻德跃教授，博士生导师，E-mail：dyyu@njau．edu．cn
