摘要
采用人工接种PRV（木瓜环斑病毒）和大田自然攻毒试验．检测了木瓜环斑病毒外壳蛋白基因转基因木瓜（PRV-CP+）和木瓜环斑病毒外壳蛋白基因──核酸酶SN基因的议价基因（PRV—CP－SN）转基因木瓜（PRVCP一SN+）对PRV侵染的抗病能力。试验结果表明，绝大多数的转基因植株对PRV的浸染表现不同程度的延缓能力，部分植株能抵抗PRV达2－3个月以上，甚至更长时间。两样本均数之差的显著性测验结果表明，PRV-CP－SN+的大田自然攻毒表现对PRV的抵抗能力比PRV－CP+的强。
PRV - CP and PRV - CP - SN transgenic papayas were detected in resistance to PRV by virtue of inoculation of PRV in greenhouse and infection of PRV in field. Results found that most of the two transgenic papayas showed delayed disease symptoms but to varying extent, some for 2 to 3 months or even more. PRV - CP-SN transgenic papayas were much more resistant to PRV than PRV - CP transgenic papayas in field trials.
出处
《热带作物学报》
CSCD
1997年第1期58-62,共5页
Chinese Journal of Tropical Crops
基金
国家"八五"攻关专题
关键词
转基因
番木瓜
人工接种
田间攻毒
抗病性
Transgenic papaya
greenhouse inoculation
field infection
PRV resistance
