摘要
建立了一种以13C6-磺胺二甲基嘧啶为内标快速测定肠衣中17种磺胺类药物残留量的方法.样品经过V（乙腈）∶V（乙酸乙酯）=1∶1 混合溶剂提取后,离心,上清液在旋转蒸发仪上蒸发至干,体积分数30%甲醇溶液定容,正己烷除去脂肪,经过滤膜后,进高效液相色谱-串联四极杆质谱进行分析,内标法定量.方法的回收率在80%～100%之间,相对标准偏差在15.4%以下,定量限为1.0 μg/kg（磺胺脒为2.0 μg/kg）,检出限在0.4 μg/kg以下（磺胺脒为1.0 μg/kg）.方法适合于大批量样品的快速检测.
A rapid method of determining 17 sulfonamides residues by using the 13^C6-sulfamethazine as an internal standard in the casing was studied by liquid chromatography-tandem mass spectrometry. Samples were extracted with 1 ： 1 acetonitrile and ethyl acetate followed by centrifuging. Then the supematant was evaporated on a rotary evaporator until dry. And 1.0 mL of solution comprised of 30： 70 methanol and pure water was added into the bottle to dissolve the matters of interest and 0.5 mL of hexane was added to remove fat. After filtration with 0.45 /an membrane, the samples were analyzed by high performance liquid chromatography and tandem mass spectrometer. The recoveies concentrated in 80% - 100%, the RSD was below 15.4%, the limit of quantitation was 1.0 μg/kg except Sulfaguanidine, which was 2.0 μg/kg, and the limit of detection was below 0.4 μg/kg （with the exception of Sulfaguanidine which was 1.0 μg/kg）.
出处
《分析试验室》
CAS
CSCD
北大核心
2007年第5期96-99,共4页
Chinese Journal of Analysis Laboratory
基金
江苏出入境检验检疫局科技项目(2005KJ16)
关键词
磺胺类药物
肠衣
液相色谱-质谱联用
多残留
Sulfonamides
Casing
Liquid chromatography-tandem mass spectrometry
Multi-residue
作者简介
吴宗贤（1981-）,男,硕士研究生
