摘要
目的研究胃癌患者外周血中(IL-6)、胃癌组织中IL-6及其受体(IL-6R)和增殖细胞核抗原(PCNA)的表达水平及它们之间的相关性,探讨三者与胃癌临床生物学指征的关系。方法采用酶联免疫吸附实验(ELISA)技术半定量、平行地检测30例胃癌患者外周血、胃癌及其相应癌旁组织中IL-6及其受体的水平,同时运用免疫组织化学法(ABC法)检测胃癌组织中PCNA的表达情况,分析IL-6、IL-6R、PCNA与胃癌临床生物学指征的相关性。结果胃癌患者血清中IL-6水平显著增高,手术后则明显下降;胃癌组织中IL-6、IL-6R水平较癌旁组织显著增高,且二者呈明显正相关;IL-6、IL-6R、PCNA在胃癌患者外周血和癌组织中的表达水平与患者肿瘤的大小、分化程度、浸润深度、有无淋巴结转移以及临床分期等临床生物学特征显著相关。结论IL-6、IL-6R和PCNA是反映胃癌细胞恶性程度和浸润转移深度的重要指标,对它们的水平进行检测,特别是对血清IL-6水平的测定,有利于指导临床诊治,判断胃癌的发生、发展和预后情况。
Objective To detect the expression of IL-6 in peripheral blood of patiems with gastric cancer, as well as the expressions of IL-6, IL-6 receptor （IL-6R） and proliferating cell nuclear antigen （PCNA） in the neoplastic tissue in order to investigate the clinical significance of such expressions in looking for a convenient and effective way for the diagnosis and therapy of gastric cancer. Methods Enzyme-linked immunosorbent assay （ELISA） was employed for the parallel semi determination of IL-6 levels in the peripheral blood of 30 patients with gastric cancer, as well as the levels of IL-6 and IL-6R in the neoplastic and adjacent tissues. The expression of PCNA in the neoplastic tissues was determined with immuno-histochemistry technique Moreover, the correlation was comprehensively analyzed among IL-6, IL-6R, PCNA and the clinicopathologic findings of gastric cancers. Results The IL-6 levels in both peripheral blood and neoplastic tissues of 30 patients were significantly higher than those of the normal controls. The IL-6 levels in peripheral blood decreased significantly after operation; the IL-6 and IL-6R levels in cancer tissues were significantly higher than those in their adjacent tissues with a positive cor relation between IL-6 and IL-6R levels. The expressions of IL-6, IL-6R and PCNA in both peripheral blood and neoplastic tissues of patients were positively related to the clinical manifestation of gastric cancer, including the differentiation degree of cancer cells, tumor size, infiltration extent and the metastasis of cancer cells. Conclusions IL-6, IL-6R and PCNA are the important factors which affect the degree of malignancy of the gastric cancer concerned, and they also, reflect the possibility of infiltration and metastasis of cancer cells. The IL-6 level in peripheral blood and the levels of , IL-6R and PCNA in tumor tissues of patients with gastric cancer are obviously high. The expression levels of all these factors are correlated with the clinicopathologic findings of gastric cancer. An adequate determination of IL-6 and PCNA is of benefit to the diagnosis and treatment of gastric cancer, and to the judgment of the course and prognosis of gastric cancer.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2007年第7期710-712,共3页
Medical Journal of Chinese People's Liberation Army
关键词
胃肿瘤
白细胞介素6
受体
白细胞介素6
增殖细胞核抗原
stomach neoplasms
interleukin-6
receptors, interleukin-6
proliferating cell nuclear antigen
作者简介
李冰，医学硕士，主治医师。主要从事胃肠道肿瘤和腹部创伤方面的研究
