摘要
目的:观察消痰散结方对小鼠胃移植瘤和瘤旁胃组织中白细胞介素8(interleukin-8,IL-8)及其受体趋化因子受体1(chemokine receptor1,CXCR1)和趋化因子受体2(chemokine receptor2,CXCR2)表达的调节作用,探讨消痰散结方抑瘤、防复发的部分作用机制。方法:50只昆明小鼠随机分为正常组、生理盐水组、清热解毒方组、喃氟啶组和消痰散结方组。除正常组外,其余各组通过移植S180瘤块,建立小鼠胃移植瘤模型。予相应药物灌胃治疗3周后,剥取肿瘤,称取瘤质量,计算抑瘤率;运用酶联免疫吸附测定法检测肿瘤及瘤旁胃组织中IL-8的蛋白表达;免疫组织化学法检测CXCR1、CXCR2蛋白表达。结果:肿瘤及瘤旁胃组织中IL-8及其受体CXCR1和CXCR2蛋白表达较正常小鼠胃组织明显升高(P<0.01);消痰散结方下调肿瘤及瘤旁组织中IL-8蛋白表达的作用优于对照药清热解毒方和喃氟啶(P<0.05);消痰散结方下调肿瘤中CXCR1蛋白表达的作用优于喃氟啶(P<0.01),对瘤旁胃组织中CXCR1蛋白表达的作用优于喃氟啶及清热解毒方(P<0.01);消痰散结方下调肿瘤中CXCR2蛋白表达的作用优于喃氟啶及清热解毒方(P<0.01),而3种药物对瘤旁胃组织中CXCR2蛋白表达的调节作用差异无统计学意义(P>0.05)。结论:消痰散结方可明显下调胃肿瘤及瘤旁胃组织中IL-8及其受体的蛋白表达,是其抑瘤、防复发的可能机制之一。
Objective： To explore the mechanisms of Xiaotan Sanjie Decoction （XTSJD）, a compound traditional Chinese herbal medicine, in inhibiting the tumor growth and preventing recurrence by testing the protein expressions of interleukin-8 （IL-8） and its receptors chemokine receptor 1 （CXCR1） and chemokine receptor 2 （CXCR2） ingastric tumor xenografts and gastric tissue adjacent to the tumor in mice.Methods： Fifty Kunming mice were randomly divided into normal group, normal saline （NS） group, Heatclearing and Detoxicating Decoction （HCDD） group, tegafur （FT-207） group and XTSJD group. Except for mice in the normal group, S180 tumor block was transplanted into the gastric walls of the mice, and the mice were administered with corresponding medicine for 3 weeks. Weight of tumor xenografts was measured and tumor inhibition rate was calculated, IL-8 protein expression was tested by enzyme-linked immunosorbent assay. Expressions of CXCR1 and CXCR2 were tested by immunohistochemical method.Results： The protein expressions of IL-8 and its receptors in tumor xenografts and gastric tissue adjacent to the tumor were markedly higher than those in the gastric tissue in normal mice （P〈0. 01）; compared with HCDD and FT-207, XTSJD could significantly decrease the IL-8 protein expression in tumor xenografts and gastric tissue adjacent to the tumor （P〈0.05）; compared with FT-207, XTSJD could significantly decrease the CXCRI protein expression in tumor xenografts （P〈0. 01）, and XTSJD could also significantly decrease the CXCR1 protein expression in gastric tissue adjacent to the tumor as compared with HCDD and FT-207 （P〈0. 01）; compared with HCDD and FT-207, XTSJD could significantly decrease the CXCR2 protein expression in tumor xenografts （P〈0. 01）, and there was no significant difference among the three drugtreated groups in CXCR2 protein expression in gastric tissue adjacent to the tumor （P〉0.05）.Conclusion： XTSJD can decrease the protein expressions of IL-8 and its receptors in tumor xenografts and gastric tissue adjacent to the tumor. It may be one of the mechanisms of XTSJDin inhibiting the tumor growth and preventing recurrence.
出处
《中西医结合学报》
CAS
2010年第1期74-79,共6页
Journal of Chinese Integrative Medicine
基金
国家自然科学基金重大研究计划资助项目(No.90709044)
上海长征医院三重三优"学科建设重大项目(2006-2010年)
作者简介
Prof. Pin-kang WEI ; Tel ： 021-81885471 ; E-mail ： czzyk@ smmu. edu. cn
