摘要
目的探讨醛固酮受体拮抗剂对腹膜透析腹膜纤维化的作用及机制。方法30只Wister大鼠随机分成3组,一组为正常组,其余两组均采用4.25%含糖透析液+脂多糖致腹膜纤维化模型。再分为对照组,安体舒通给药组,30天后处死各组大鼠,留取腹膜组织做HE及Masson染色,留取血液和腹透液做ELISA法测TGF-β,腹透液计数腹水中细胞总数和巨噬细胞数,免疫组织化学法测定腹膜组织MCP-1,C-J。结果安体舒通组的壁层腹膜厚度比模型组薄,腹水细胞总数和巨噬细胞数减少(P<0.01),转化生长因子-β的浓度下降,MCP-1表达下调(P<0.01)。结论醛固酮受体拮抗剂安体舒通可明显抑制腹膜间皮细胞MCP-1表达,降低TGF-β活性,抑制腹膜炎症,从而减轻腹膜纤维化。
Objective To investigate the effect and possible mechanism of aldosterone receptor blocker on peritoneal fibrosis. Methods 30 male Wistar rats were divided into three groups at random, Group A （n = 10） control group,B,C（n = 10） received daily intraperitoneal injection of 20 mL 4.25% Beter dialysate. on day 1, 3 ,5 ,7,The rats was given intraperitoneal injection of LPS 0.6mg/kg C（n= 10）medicine group, Aldosterone eceptor blocker. 100mg/kg/day was given orally to rats by gastric gavage in the morning once a day. Thirty days later all rats were sacrificed.the parietal peritoneum was stained with HE and.Masson. Total cell counts and macrophage counts in dialysate. To measure TGF-βof blood and abdominal cavity liquid of peritoneal dialysis rats. With ELISA method,and to measure the express of abdominal membrane MCP-1 with immunological histochemical method. Results TGF-β obviously increased in Both B and C groups as compared to A group （P 〈 0.01） ,group B was more serious than group C （P〈 0. 01）, so do histopathology of peritoneum. Conclusions Aldosterone eceptor blocker functions as an inhibitor of TGF-βexpression in blood and dialysate and suppressed MCP-1 expression in abdominal membrane. It demonstrates there is Aldosterone eceptor on abdominal membrane at the same time.
出处
《齐齐哈尔医学院学报》
2007年第7期774-776,共3页
Journal of Qiqihar Medical University
关键词
醛固酮受体拮抗剂
腹膜纤维化
转化生长因子-Β
单核细胞化学激动蛋白-1
Aldosterone receptor blocker Peritoneal fibrosis Transform growth factor-β Monocyte chemoattractant protein-1 Activator protein-1
作者简介
哈尔滨医科大学在读硕士（赵向阳）
