摘要
2004年4月采集大连金州湾沿岸畜禽养殖场猪、牛、鸡和某中学人粪便样品。经改良的生化鉴定方法鉴定,得大肠埃希菌(Escherichia coli)猪源75株、牛源78株、鸡源69株、人源68株,占粪大肠菌比率分别为85.23%、92.86%、80.23%、80.00%。为验证改良的生化鉴定方法准确性,从中随机选取8株E.coli,扩增16S rRNA序列并与Genebank中E.coli16S序列比对,确定这8株细菌与E.coli同源相似率达99%以上,进而验证改良的生化鉴定方法的可行性。
Feces samples of swine, cattle, fowl from farms, and human from a middle school were collected from coastal area of.Iinzhou Bay, Dalian City, April 2004. Through improved biochemical identification method 88, 86, 84, and 85 fecal coliform were isolated from the above-mentioned relevant sources respectively. 75 E. coli strains were from swine, 78 from cattle, 69 from chicken, and 68 from human respectively. The rates of E. coli over fecal coliform were 85.23% , 92.86% , 80.23% , and 80.00% respectively. In order to test the veracity of the results, eight E. coli strains randomly from the four sources were amplified 16S rRNA sequence and compared with 16S sequence of E. coli from Genebank. The resembling rates from the same source can exceed 99% , therefore, the improved biochemical identification method is feasible.
出处
《微生物学杂志》
CAS
CSCD
2007年第3期1-5,共5页
Journal of Microbiology
基金
国家高技术研究发展计划项目(863项目
2003AA635180)
作者简介
杨滴男，硕士研究生。主要从事环境微生物生态学研究。
通讯作者
