摘要
目的研究酵母菌HY-05发酵生产辅酶Q10提高产量的方法。方法研究不同初糖浓度及补加方式,补加玉米浆和控制溶氧对酵母菌发酵过程中菌体量和辅酶Q10产量的影响,确定辅酶Q10补料分批发酵的最佳条件。结果酵母菌HY-05产辅酶Q10的量达到156.2 mg/L,与不补料相比产量提高了58.1%。结论此法能提高酵母菌HY-05发酵生产辅酶Q10的产量。
Objective To study the method of enhancing the yield of CoQ10 by fermentation from yeast HY-05. Methods The effects of different initial sucrose concentration, feeding mode of sucrose, feeding of corn steep liquor and dissolved oxygen on some parameters, such as yield of CoQ10, biomass of yeast, during the fermentation process of CoQ10 were studied. The optimum condition of fed-batch fermentation of CoQ10 was confirmed. Results Under these optimum condition, the yield of CoQ10 reached to 156.2 mg/L, which were 58.1% higher compared with the fermentation without feeding sucrose and corn steep liquor. Conclusion This method can enhance the yield of CoQ10 by fermentation from yeast HY-05.
出处
《食品与药品》
CAS
2007年第06A期1-5,共5页
Food and Drug
关键词
辅酶Q
补料分批发酵
条件优化
coenzyme Q
fed-batch fermentation
optimal condition
作者简介
肖海蓉（1980-），女，硕士，研究方向为药物生物合成E-mail：happyxhr@yahoo．com．cn
通讯作者：许激扬（1964-），男，副教授，研究方向为药物生物技术E-mail：jiyangx@126．com
