摘要
目的研究不同浓度雌二醇（E2）对去卵巢小鼠脾细胞雌激素受体（ER）及辅助性T细胞1（Th1）和辅助性T细胞2（Th2）之间平衡的影响。方法将小鼠脾细胞分为7组：青年组、假手术组、去卵巢对照组及去卵巢＋E2（10^-11mol/L、10^-10mol/L、10^-9mol/L、10^-8mol/L）组。逆转录-聚合酶链反应（RT-PCR）方法检测ERα、ERβmRNA的表达;酶联免疫吸附法（ELISA）法检测细胞因子干扰素-γ（IFN-γ）、白细胞介素-2（IL-2）和白细胞介素-4（IL-4）水平,以了解Th1与Th2的平衡关系。结果去卵巢对照组ERα、ERβmRNA水平分别为0．68±0．15和0．70±0．17,较青年组（0．95±0．21和0．93±0．1 9）及假手术组（0．98±0．28和0．94±0．20）下降（P＜0．05和P＜0．01）;去卵巢＋E2（10^10mol/L）组ERα、ERβ的表达分别为0．95±0．19和0．90±0．29,较去卵巢对照组升高（P＜0．05和P＜0．01）。去卵巢对照组IFN-γ、IL-2分别为（360．36±8．64）ng/L和（1485．49±5．01）ng/L,较青年组[（93．46±4．81）ng/L和（704．37±5．32）ng/L]及假手术组[（92．47±2．95）ng/ L和（716．84±1 6．48）ng/L]升高（均为P＜0．01）;而去卵巢对照组IL-4水平[（258．11±6．00）ng/L]低于青年组[（269．77±8．44）ng/L]和假手术组[（270．18±10．34）ng/L]（均为P＜0．05）。去卵巢＋ E2（10^10mol/L）组的IFN-γ、IL-2分别为（94．05±7．17）ng/L和（712．15±13．84）ng/L,低于去卵巢对照组（均为P＜0．01）,IL-4为（269．76±2．05）ng/L,高于去卵巢对照组（P＜0．05）。结论不同浓度E_2对去卵巢小鼠脾细胞ERα、ERβ基因表达起不同的调节作用：在生理范围内为正向调节,超生理范围为负向调节。小鼠去卵巢后影响脾细胞Th1和Th2的平衡,表现为Th1漂移。生理浓度的E2能部分逆转Th1漂移,部分恢复Th1和Th2的平衡。
Objective To investigate the effects of different concentrations of estradiol on estrogen receptor expression and T-helper 1/ T helper 2 （Thl/Th2） balance of splenocytes of ovariectomized mice. Methods The mice splenocytes were divided into seven groups： young group, sham-ovariectomized group, ovariectomized group and four ovariectomized plus estradiol groups （estradiol concentration： 10^-11mol/L, 10^-10 mol/L, 10^-9 mol/L, 10^-8 mol/L）. The estrogen receptor α （ERα）, estrogen receptor β （ERβ） mRNA expressions were determined by reverse transcriptase polymerase chain reaction （RT PCR）. The levels of interferon-γ （IFN-γ）, interleukin-2 （IL-2） and interleukin 4 were evaluated by enzyme linked immunosorbent assay （ELISA）, which implied Th1/Th2 balance. Results ERα and ERβ mRNA expressions were significantly lower in ovariectomlzed group（0.68±0.15,0.70±0.17） than those in young group （0.95±0.21,0.93±0. 19）and sham-ovariectomized group（0.98±0.28,0.94±0.20） （P〈0.05, P〈0.01）. Compared with ovariectomized group, ERα, ERβ mRNA expressions in ovariectomized plus estradiol（10^10 mol/L） group（0.95±0.29,0.90±0.29） increased （P〈0.05,P〈0.01）. IFN-γ and IL-2 levels in ovariectomized group（360.36±8.64,1485.49±5.01） were extremely higher than those in young group（93.46±4.81,704.37±5.32） and sham-ovariectomized group （92.47±2.95,716.84±16.48） （all P〈0.01）. However, IL-4 level inovariectomized group（258.11±6.00） was lower than that in young group （269.77±8.44）and in sham ovariectomized group （270. 18±10.34） （all P〈0. 05）. The IFN-γ and IL-2 levels in ovariectomized plus estradiol（10^-10 mol/L） group（94.05±7.17,712.15± 13.84） were significantly lower than those in ovariectomized group （P 〈 0.01）, but IL-4 level （269.76±2.05） was higher than that in ovariectomized group（P〈0.05）. Conclusions Different levels of estradiol have different effects on ERα, ERβ gene expressions in splenocytes of ovariectomized mice： ERs expressions are up regulated at physiological dose of estrogen and down-regulated at higher doses. In ovariectomized mice, the Th1/Th2 balance is destroyed, which suggests a Th1 polarization. Physiological doses of estrogen could partially decrease Thl type cytokine and restore the Th1/Th2 balance.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2007年第5期379-382,共4页
Chinese Journal of Geriatrics
基金
基金项目：上海市科委基础研究重点项目（04JC1492）
上海市医学重点学科（妇产科学）建设项目（05-Ⅲ-0165）
作者简介
通讯作者：张绍芬，Email：zhangshaofen@163．com
