摘要
目的:观察逆灸关元穴对不同月龄更年期大鼠血清和子宫肿瘤坏死因子α及脾脏雌激素受体α表达的影响,以及逆灸对更年期大鼠免疫功能的影响及脾脏雌激素受体α的调节机制。方法:实验于2003-11/2004-06在北京中医药大学针灸学院针灸机理实验室完成。选用健康SD雌性大鼠128只,4月龄16只,体质量(250±20)g,为4月龄对照组。10月龄112只,体质量(300±20)g,按照随机数字表法分为7组,每组16只,分别为12,14,16月龄逆灸组,10,12,14,16月龄对照组。采用自然老化方式进行实验。逆灸组大鼠均在10月龄开始艾炷灸(小号),灸前暴露关元穴区(鼠后下肢根部在下腹连线的中点)直径约1.5～2.0cm区域皮肤,轻抓大鼠,让其仰躺在手掌上,将艾炷点燃后,直接放置在关元穴上施灸,每次灸一壮。灸后局部皮肤略潮红。灸2次/周,共灸8周。4,10,12,14,16月龄对照组除同逆灸组轻抓仰躺外不做任何处理。大鼠于4,10,12,14,16月龄采用放射免疫法测定血清、子宫肿瘤坏死因子α;采用免疫组织化学法测定脾脏雌激素受体α。结果:纳入动物128只,均进入结果分析。①与4月龄对照组比较,10,12,14,16月龄对照组大鼠血清肿瘤坏死因子α呈先降后升趋势,16月龄对照组明显高于4月龄对照组,差异有显著性意义[分别为(2.598±0.830),(1.826±0.379)μg/L,P<0.05];子宫肿瘤坏死因子α为先升后降趋势,差异均有显著性意义(P<0.05);与同月龄对照组比较,各逆灸组血清肿瘤坏死因子α呈先升后降趋势,即12,14月龄上升,其中12月龄逆灸组明显高于12月龄对照组,差异有显著性意义[分别为(2.755±0.357),(1.290±0.251)μg/L,P<0.05],16月龄逆灸组明显低于16月龄对照组[分别为(1.692±0.238),(2.598±0.830)μg/L,P<0.05];子宫肿瘤坏死因子α则12,16月龄逆灸组明显高于12,16月龄对照组,差异均有显著性意义[分别为(15.329±4.613),(10.730±1.366)μg/g;(11.622±1.884),(8.814±1.405)μg/g,P<0.05],14月龄略下降(P>0.05)。②与4月龄对照组比较,10,12,14,16月龄对照组大鼠脾脏雌激素受体α的阳性表达(A)呈波动性下降,其中16月龄对照组明显低于4月龄对照组(分别为9384.605±446.939,12813.915±770.039,P<0.01)。与同月龄对照组比较,各逆灸组脾脏雌激素受体α的阳性表达(A)比各对照组均上升,其中12,16月龄逆灸组明显高于12,16月龄对照组,差异有显著性意义(分别为16233.762±519.358,11294.618±350.804;11195.763±431.613,9384.605±446.939,P<0.01,0.05)。结论:更年期大鼠随月龄增加,免疫因子肿瘤坏死因子α已出现紊乱。逆灸可通过平抑此期血清肿瘤坏死因子α异常波动,提高子宫肿瘤坏死因子α含量和脾脏雌激素受体α的表达水平以达调节大鼠更年期免疫功能。并这种调节效应凸显于12月龄和16月龄。
AIM： To observe the content of tumor necrosis factor alpha （TNF-α） in serum and uterus and the expression of estrogen receptor alpha （ER-α） in spleen of menopausal ruts by preventive moxibustion （PM） on Guanyuan （RN 4）, and explore the effect of PM on immunological function and the mechanism of PM on regulating expression of ER-α in spleen of rats during menopause.
METHODS： From November 2003 to June 2004, the experiment was completed in the laboratory of School of Acupuncture, Beijing University of Chinese Medicine. A total of 128 SD female rats were adopted and divided into two sets： One was the four month-aged group, containing 16 rats with （250±20） g weight, The other was ten month-aged group, containing 112 rats with （300±20） g weight. Each set included 16 rats and were divided into seven groups by random number： 12, 14 and 16 month-aged PM groups and 10, 12, 14 and 16 month-aged control groups. Natural aging model was used in the experiment. In PM groups, moxibustion with moxa cone was directly applied to rat＇s Guanyuan （RN 4） around the skin of 1.5-2.0 cm diameter from the 10^th month （one acupuncture every time, twice a week for eight weeks continuously）. And it remanded that the local skin turned red slightly after moxibustion. The controlled rats were grasped gently and made to lie on their backs, which was the same with PM groups. Radio-immunoassay and immunohistochemical method were applied to determine the content of TNF-α in serum and uterus and the expression of ER-α in spleen of rats at their age of 4, 10, 12, 14 and 16 months.
RESULTS： Totally 128 rats were involved in the result analysis. （1）With the age increased, the content of TNF-α in serum first decreased and then increased in all groups, and obvious differences appeared in the control group at the 16^th month [（2.598±0.830）, （1.826±0.379）μg/L, P 〈 0.05]; While the content of TNF-α in uterus tended to first increase and then decrease, with the significant differences （P 〈 0.05）. Compared with the control groups, the content of TNF-α in serum first increased at months 12 and 14, and then decreased at month 16 in PM groups, and obvious differences appeared at months 12 and 16 [（2.755±0.357）, （1.290±0.251）μg/L, P 〈 0.05; （1.692±0.238）, （2.598±0.830）μg/L, P 〈 0.05]; The content of TNF-α in uterus increased in PM groups at months 12 and 16 [（15.329±4.613）, （10.730±1.366）μg/g; （11.622±1.884）, （8.814±1.405）μg/g, P 〈 0.05], and a little decreased at the age of 14 months （P 〉 0.05）.（2）The positive expression of ER-α in spleen had a fluctuating downtrend, and obviously lower in the control groups at month 16 than at month 4 （9 384.605±446.939, 12 813.915±770.039, P 〈 0.01）. Compared with the control groups, the positive expression of ER-α in spleen increased significantly in the corresponding PM groups, especially at months 12 and 16 （16 233.762±519.358, 11 294.618±350.804; 11 195.763±431.613, 9 384.605±446.939, P 〈 0.01,0.05）.
CONCLUSION： With the ages increases, immune factor TNF-α of rats during menopause appears disorder. PM can raise the content of TNF-α in uterus and the expression of ER-α in spleen to regulate immunity of menopausal rats by preventing abnormal fluctuation of TNF-α in serum. And the regulation effect is more obvious at the age of 12 and 16 months.
出处
《中国临床康复》
CSCD
北大核心
2006年第43期171-173,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金课题(90209026)
北京市自然基金课题(7052037)~~
作者简介
李晓泓☆，女。1960年生，北京市人，汉族．2004年北京中医药大学毕业，博士，教授，博士生导师．主要从事针灸与治未病相关性的研究。
