摘要
以渝桉1号的单芽茎段为外殖体进行组织培养技术研究.结果表明,渝桉1号的启动诱导培养基以EU+BA2.0 mg.L-1+NAA 0.1 mg.L-1较为适宜.继代增殖以EU+BA 1.0 mg.L-1+NAA 0.2 mg.L-1的培养基配方为宜,每个芽可产生5个芽.生根较适培养基为1/2MS附加NAA 0.2 mg.L-1或IBA 0.2 mg.L-1,生根率95%以上.试管苗移栽成活率达到91%以上.
Using stem-segments with a single bud as explants, in vitro culture and rapid propagation were performed of Eucalyptus uorphylla X E. grandis ＇ CW-1 ＇. The results showed that the optimum medium for bud regeneration was EUq-BA 2.0 mg·L^-1＋NAA＇0.1 mg·L^-1. The optimum subculture medium was EUq-BA 1.0 mg·L^-1＋NAA 0. 2 mg·L^-1, on which each explant produced up 5 plantlets on the average. The regenerated shoots rooted successfully on 1/2MS supplemented with NAA 0.2 mg ·L^-1 or IBA 0. 2 mg·L^-1 , with a rooting rate of 95%. Over 91% of the tube plantlets survived after transplanting.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第4期67-69,共3页
Journal of Southwest University(Natural Science Edition)
基金
重庆市科委攻关项目(2006AC1061)
重庆市教委重点科研项目
关键词
桉树
离体培养
快速繁殖
植株再生
Eucalyptus urophylla
in vitro culture
rapid propagation
plant regeneration
作者简介
杨帆 （1963）男，重庆江津人，副教授，硕士，主要从事园林植物的科研和教学工作研究．
