摘要
应用正交设计法研究BA,NAA及蔗糖3个因子对牛蒡(ArctiumlappaL.)组培苗的增殖率的影响,同时考察了NAA,BA及不同浓度的MS培养基3个因子对牛蒡组培苗生根培养的影响,各试验均按正交表L9(34)形成3因素、3水平的不同配比。试验结果表明:最佳的增殖培养基是MS+BA2.0mg/L+NAA0.05mg/L+蔗糖30g/L,最适于生根的培养基是1/2MS+BA0.1mg/L+NAA0.05mg/L+30g/L蔗糖。
In order to analyze the effect of different contents of hormones and sucrose in the medium on tufted-bud induction and tufted-bud rooting of Arctium lappa L., tissue culture of the plant with the seeds as the explants was carried out, in which the orthogonal design, L_9(3~4), was used. The results indicated that MS supplemented with BA 2.0 mg/L + NAA 0.05 mg/L + sucrose 30 g/L was suitable for its multiplication and1/2MS supplemented with BA 0.1 mg/L + NAA 0.05 mg/L + sucrose 30 g/L was optimal for rooting.
出处
《西南农业大学学报（自然科学版）》
CSCD
北大核心
2004年第6期696-698,702,共4页
Journal of Southwest Agricultural University
