摘要
目的研究淋巴瘤与反应性增生淋巴结的基因表达差异,并对淋巴瘤高表达基因FLJ39061进行初步分析。方法将淋巴瘤淋巴结标本和反应性增生淋巴结标本液氮速冻切片,使用激光显微切割技术分离淋巴细胞,提取淋巴细胞中的mRNA与表达谱芯片杂交,通过信号扫描、处理后获得两者的表达差异基因。用生物信息学方法对一条有显著性差异的新基因FLJ39061进行初步分析。结果表达谱芯片发现了152条差异表达基因。对淋巴瘤相关基因FLJ39061的性质进行了有效的预测,基本明确了该基因编码蛋白为一核蛋白,很可能是人MAP激酶激活的蛋白激酶2的前体或异构体。结论FLJ39061可能作为激酶磷酸化底物调节细胞代谢,与淋巴瘤发生有关,可作为治疗靶点做进一步的功能研究。
Objective To explore the differential expressed genes in lymphoma and reactive lymph node and performed an initial analysis on a novel gene. Hypethetical Protein FIJ39061 ,which is highly expressed in lymphnode of lymphoma. Methods The samples were collected and flash frozen quickly to maintain the integrity of mRNA. The mRNA of the tissue from the lymphoma and reactive lymph node are marked with biotin respectively and hybridized with expression profile microarray, and the differential expressed genes are obtained. Initial bioinformatics analysis is performed on a novel gene named FLJ39061 which have no functional researches at present. Its gene structure, genomic localization, the physical and chemical characteristics of the putative protein, subcellular localization, functional domain and so on are predicted, and the systematic evolution analysis is performed on the similar proteins among several species. Results Using expression profile microarray, 152 differential expressed genes are uncovered. Efficient bioinformatics analysis have fundamentally identified that FLJ39061 is a nuclear protein, and probably is precursor or isomer of MAP kinase-activated protein kinase 2. Conclusion By bioinformatics analysis, FLJ39061 may involved in the phosphorylation regulation. It is anticipated that this project will advance our understanding of the molecular mechanisms in the neoplastic transformation of lymphoma and the new insights will help to identify promising molecular targets for therapeutic intervention.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2007年第4期241-244,共4页
Cancer Research on Prevention and Treatment
基金
国家重大基础研究发展资助项目(973项目)(2002CB513100)
国家自然科学基金资助项目(C03020705)
作者简介
严玲玲（1980-），女，在读硕士，主要从事肿瘤侵袭和转移的分子机制方面的研究
通讯作者：马丁
