摘要
目的研究慢性脑血流低灌注对大鼠血和脑内β-淀粉样肽(β-amyloid peptide,Aβ)含量的影响。方法老年(10月龄)雄性SD大鼠双侧颈总动脉结扎,分别于手术后10d、30d、90d和180d进行Morris水迷宫实验;用放射免疫分析法检测血清、大脑皮质及海马的Aβ含量;用Western blot方法检测大脑皮质和海马的β-淀粉样肽前体蛋白(β-amyloid precursor protein,APP)的含量。结果Morris水迷宫实验显示,大鼠在术后30d、90d、180d均出现学习记忆功能下降,与对照组比较差异有统计学意义(P<0.05);血清Aβ水平在术后10d显著增高(P<0.01),30d、90d、180d血清Aβ水平仍高于对照组(P<0.05);术后90d和180d海马的Aβ和APP增高(P<0.05),180d皮质的Aβ和APP增高(P<0.05)。结论慢性脑血流低灌注引发大鼠脑及血中Aβ的升高,可能在阿尔茨海默病(Alzheimer’s disease,AD)早期发病过程中有重要意义。
Objective To investigate the changes of β-amyloid peptide（Aβ） in rats＇ blood and brain under the condition of chronic cerebral hypoperfusion. Methods Cerebral blood hypoperfusion rat model established by bilateral common carotid artery permanent ligation was used. Learning memory function were examined by Morris water maze. Radioimmunoassay and western blotting were employed to test the levels of Aβ and β-amyloid precursor protein （APP） in serum, cortex and hippocampal. Results The deficiency in learning-memory function began to appear from 1 m after hypoperfusion. Levels of Aβ in serum was significantly increased in each experimental group. Both APP and Aβ were increased from 3 m after hypoperfusion in hippocampal and the same changes occurred from 6m after hypoperfusion in cortex. Conclusion Chronic cerebral hypoperfusion can lead to increase of Aβ both in brain and blood which may play a very important role in early pathogenesis of Alzheimer＇s disease（AD）.
出处
《首都医科大学学报》
CAS
2007年第2期234-238,共5页
Journal of Capital Medical University
基金
北京市教育委员会科技发展计划面上项目(200410025011)资助项目
作者简介
通讯作者
