摘要
20世纪70年代初期。法国科学家报道了杂多阴离子[SiW12O40]^4-的抗病毒活性，以后相继发现了[BW12O40]^5-[P2W12O40]^6-等在体外也表现出抗病毒活性。80年代后期。具有抑制艾滋病病毒作用的（NH4）17Na[NaSb9W21O86]·14H2O（HPA-23）被应用于临床试验回。虽然其治疗效果不够理想．但却激发了人们对多金属氧酸盐的研究兴趣。90年代以来．Yamase等研究了十几种多金属氧酸盐的抗肿瘤、抗爱滋病毒活性，发现K7[PTi2W10O40]·6H2O（PM-19）具有较强的抗HIV病毒能力和较低的毒性。此后多金属氧酸盐的药物化学迅速发展起来。
Under the imitated physiological condition of animal body, the interactions of heteropoly salt （PM-19） with bovine serum albumin （BSA） were investigated by fluorescence and absorption spectroscopy. It was shown that this compound had a quite strong ability to quench the fluorescence launching from BSA. After analyzing the fluorescence quenching data according to Stern-Volmer equation and Lineweaver-Burk double-reciprocal equation, we found that BSA had reacted with PM-19 and formed a certain new compound. The quenching belonged to static fluorescence quenching. According to Lineweaver-Burk equation, the forming constants of the compound （298 K： 2.68 × 10^5 L ·mol^-1; 304 K： 2.19 × 10^5 L ·mol^-1; 310 K： 1.82 × 10^5 L ·mol^-1） and the thermodynamic parameters （△H=-24.72 k J·mol^-1; △S=20.97 J·mol^-1·K^-1 / 20.92 J·mol^-1·K^-1 / 20.97 J·mol^-1·K^-1; △G=-30.97 kJ·mol^-1 / -31.08 kJ· mol^-1 / -31.22kJ· mol^-1） at the corresponding temperatures were obtained. The latter shows that binding power between them is mainly electrostatic interaction. Based on Foerster＇s non-radiation energy transfer mechanism, the binding locality （r=4.14 nm） was calculated between donor and accepter. The effect of PM-19 on the conformation of BSA was also analyzed by synchronous fluorescence spectroscopy.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
2007年第4期688-692,共5页
Chinese Journal of Inorganic Chemistry
基金
国家自然科学基金资助项目(No.20271024)
关键词
杂多酸盐
牛血清白蛋白
荧光光谱
紫外光谱
热力学参数
同步荧光光谱
heteropoly salt
BSA
fluorescence spectroscopy
absorption spectroscopy
thermodynamic parameter
synchronous fluorescence spectroscopy
作者简介
第一作者：梁彦秋，女，35岁，副教授；研究方向：生物无机化学和分子光谱。
通讯联系人。E-mail：dalin0417@163．com;Tel：024-62207819
