摘要
用同步荧光法并辅以普通荧光法对不同浓度稀土离子及pH诱导的牛血清白蛋白 (BSA)构象变化进行了详细研究 ,发现稀土离子使色氨酸 (Trp)残基的荧光蓝移 ,荧光强度降低 ;酪氨酸 (Tyr)残基荧光峰位置不变 ,稀土离子浓度较低时 ,荧光峰强度降低 ,而当浓度较高时 ,Tyr残基荧光峰强度反而增强。据此推断了稀土离子与BSA结合反应中Trp残基微环境和Tyr残基微环境及构象的变化并与pH引起的变化进行了比较。
The conformational change of BSA induced by rare earth ions and pH were studied by synchronous fluorescence spectroscopy firstly. The results show that the synchronous fluorescence spectra of Trp have blue shift and are quenched in the presence of rare earth ions. While without obvious shift to Tyr, the fluorescence intensity of Tyr decreases when the concentration of rare earth ions is lower. However it increases when the rare earth ions concentration becomes higher. At last, the change of Trp, Tyr microenvironment and BSA conformation is induced by rare earth ions and pH is deduced. The functional difference between rare earth ions and pH was discussed.
出处
《中国稀土学报》
CAS
CSCD
北大核心
2004年第3期393-397,共5页
Journal of the Chinese Society of Rare Earths
基金
河北省自然科学基金 ( 2 0 0 15 3 )和河北省教育厅博士基金资助项目
关键词
分析化学
构象变化
同步荧光
牛血清白蛋白
稀土
chemical analysis
conformational change
synchronous fluorescence spectrocopy
bovine serum albumin
rare earths
