摘要
从肿瘤组织中分离出ＴＩＬ，经ｒＩＴ－２培养扩增后进行自体回输，输注途径包括：（１）外周静脉；（２）局部（肿瘤供血动脉或腹腔）；（３）静脉加局部。ＴＩＬ输注总量为１．５×１０＾９以上。治疗前后抽取外周静脉血检测Ｔ细胞亚群和ＮＫ细胞活性。１８例患者（胃癌９例、大肠癌５例、肝癌４例）经ＴＩＬ治疗后，８８．９％的患者ＮＫ活性增强，７７．７％￣９４．４％的患者ＣＤ３＋、ＣＤ４＋和ＣＤ８＋细胞比例升高。
TILs which were isolated from tumor tissue, and cultured with rIL - 2 for expansion were autoinfused by way of (1) peripheral vein; (2)local (blood supplying artery for tumor or peritoneal cavity); (3)vein plus local, at a total amount of more than 1.5×109. Before and after treatment, peripheral blood was taken for the detection of T subgroup cells and NK activity. 18 cases (gastric Ca 9, colonic Ca 5, liver Ca 4) were treated with TIL, of whom NK activity was enhanced in 88.9%,CD3+ , CD4 + , and D8+ subgroup were elevated in 77.7%-94.4%, showing different ways of infusion had a different effect on immunologic function. It has been suggested that TIL in vivo by way of venous infusion can secreat cytokine to enhance the immunologic function. However it is the best way to combine local infusion with venous one for enhancement of immunologic function as well as concentration of TIL in tumor tissue.
出处
《外科》
1996年第3期89-91,共3页
