摘要
采用顶空气相色谱法对绿茶提取物中乙酸乙酯残留溶剂的分析方法进行了研究。试验采用大口径HP-快速GC残留溶剂柱为分离柱，对顶空平衡温度、平衡时间对残留有机溶剂乙酸乙酯测定的影响进行了分析研究。结果表明：乙酸乙酯在0．0765-0．612μg／ml内线性关系良好，相关系数为0．9993；方法精密度试验RSD（n=6）为3．60％；平均回收率范围95．42％～97．88％，RSD1．95％～3．09％；检测限（SIN=3）为0．005μg／ml。本方法快速、灵敏、准确、无污染，适用于绿茶提取物中乙酸乙酯残留量的测定。
A method for the determination of residual ethyl acetate in green tea extract has been studied by headspace gas chromatography in order to set up an analysis method. A wide bore HP-fast GC residual solvent column was performed, and the effects of the equilibrium temperature and equilibrium time on the determination of ethyl acetate were studied. The results showed that the standard curve was linear in the range of 0.0765-0.612μg/ml （r=0.9993）. the relative standard deviation （n=6） of precision was 3.60%. the average recoveries were between 95.42% and 97.88% with RSD in the range of 1.95%-3.09%, the minimum detectable concentration（S/N=3） was 0.005 μg/ml. It is a rapid, sensitive, accurate and uncontaminated method and applicable for the determination of residual ethyl acetate in green tea extract.
出处
《茶叶科学》
CAS
CSCD
北大核心
2007年第1期67-70,共4页
Journal of Tea Science
基金
上海市自然科学基金(05ZR14088)
上海市重点学科建设项目(T0503)
上海市联盟计划-难题攻关项目
关键词
顶空气相色谱法
绿茶提取物
乙酸乙酯
残留有机溶剂
headspace gas chromatography, green tea extract, ethyl acetate, residual organic solvent
作者简介
胡慧廉（1963-），女，工程师，硕士，从事色谱分析研究。E-mail：huhl@ecust．edu．cn
