摘要
研究了尼罗罗非鱼(Oreochromis niloticus)肝脏淀粉酶的基本性质及金属离子对该鱼淀粉酶活性的影响。结果表明:罗非鱼淀粉酶活力的最适pH是6.5,最适底物浓度是2%。研究金属离子对该鱼淀粉酶活力影响,一价金属离子K+、Li+、Na+对酶活力影响较小;二价金属离子Cu2+对酶活力具有抑制作用,Zn2+对酶活力无明显影响;三价金属离子Al3+对酶活力具有抑制作用,但效果不是很强烈,Fe3+对酶活力具有明显的激活作用;碱土金属Mg2+、Ca2+对淀粉酶活性有激活作用,而Ba2+对酶具有抑制作用;重金属离子Cd2+、Pb2+有明显的抑制作用。
Properties of amylase from Nile tilapia （Oreochromis niloticus ） was studies in this paper. The result showed that： the optimum pH for amylase was 6. 5, the optimum substrate concentration was 2%. The effects of some metal ions on the amylase activity were studied. And the positive univalent metal ions like K^＋ , Li ^＋ and Na^＋ had little influence on the enzyme activity. The positive bivalent metal ions inhibit the enzyme and the order of the inhibit intensity was as follows ： Cu^2＋ 〉 Zn^2＋. And positive bivalent alkaline earth metalions （ Mg^2＋ , Ca^2＋ and Ba^2＋ ） affected the enzyme and the order of the activation intensity was as follows ： Mg^2＋ 〉 Ca^2＋ , but Ba^2＋ inhibit the enzyme activity. The positive trivalent metal ions Al^3＋ had the inhibitory activity, but the effect was unobvious, and Fe^3＋ had the activation effect. And heavy metal ions Cd^2＋ , Pb^2＋ inhibited the enzyme strongly.
出处
《淡水渔业》
CSCD
北大核心
2007年第2期34-37,共4页
Freshwater Fisheries
基金
泉州师范学院重点学科(XK0609)
大学生科研基金(2006KJ24)
作者简介
谢进金（1965-），男，福建安溪人，主要从事动物生理生化等方面研究。E-mail：xiejinjin@qztc．edu.cn
