摘要
报道了中药常山沸水浸取液的荧光光谱。在三维荧光等高线光谱图中出现3个喹唑啉类生物碱（常山的主要活性成分）的荧光峰，其激发波长分别为235，270和320nm，发射波长均为430nm。三维荧光等高线光谱是一种形象的指纹图谱，可以作为中药定性分析的依据。在pH3～6范围内，常山水浸液二维荧光激发光谱的形状随pH的变化而改变，推测是由于喹唑啉类生物碱分子中喹唑酮环上N-1的质子化所致。在近中性条件下，常山水浸液的荧光强度与溶液浓度之间有良好的线性关系，可以作为喹唑啉类生物碱定量分析的基础。
Fluorescence spectra of chang shan （Dichroa febrifuga Lour） aqueous extraction were studied. In the three-dimensional fluorescence contour spectrum, three fluorescence peaks of quinazoline alkaloids, which are the active components of changshan, were observed. The excitation wavelengths of the peaks were 235, 270 and 320 nm, respectively, and the emission wave- length of all the peaks was 430 nm. Three-dimensional fluorescence contour spectrum is the very image of fingerprint, suitable for qualitative identification of traditional Chinese medicine. In the range of pH 3 to pH 6, the fluorescence spectrum of chang shan aqueous extractions changes with the variation in pH value. The reason for this spectral change might be the protonation of N-1 in quinazolone ring of β-dichroine （febrifugine） molecule. There is an excellent linear relationship between the fluorescence intensity and the concentration of chang shan under nearly neutral conditions, thereby a quantitative method for the determination of quinazoline alkaloids may be established.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2007年第2期342-345,共4页
Spectroscopy and Spectral Analysis
基金
河北省自然科学基金项目(B2005000149)
河北师范大学科研基金项目(L2004204)资助
关键词
常山
喹唑啉类生物碱
荧光光谱
中药指纹图谱
Dichroa febrifuga
Quinazoline alkaloids
Fluorescence spectra
Fingerprint of traditional Chinese medicine
作者简介
张英华，女，1980年生，河北师范大学化学学院硕士研究生
通讯联系人e-mail：weiyju@heinfo．net
