摘要
为了研究水稻根负向光性的光受体,观察了光质对稻根的负向光性效应,用紫外-可见分光光度计测定了水稻根冠浸提液的吸收光谱,并对根冠中的蛋白质进行了SDS-PAGE电泳分析。蓝紫光能显著诱导稻根负向光性,而红光无效;根冠浸提液的吸收光谱在350nm和450nm各有一个吸收峰;根冠中有120kD的光受体特征的蛋白条带。推测稻根负向光性的光受体可能为蓝光受体。
To properly explore the photoreceptor for the negative phototropism in rice (Oryza sativa L. ) root, lights with different wavelengths were applied to investigate the effect of light quality on phototropic response. The negative phototropic bending could be induced prominently by blue/UV light, while not be induced by red and far-red light. Absorption spectrum of the extracted solution from rice cap had two peaks under 350 nm and 450 nm accordingly, and the molecular weight of the 120 kD protein of the cap under unilateral light was larger than that under the dark. It suggested that the blue light receptor might be the photoreceptor for the negative phototropism in rice root.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2007年第2期155-158,共4页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目(30270795)
植物生理学和生物化学国家重点实验室开放课题基金资助项目(2002001)
江苏省高校研究生科技创新计划基金资助项目
关键词
水稻
负向光性
根
光质
光受体
rice
negative phototropism
root
light quality
photoreceptor
作者简介
汪月霞(1980-),女,博士研究生,E-mail:yueer128056@126.com。
通讯联系人,E-mail:wangzhong@yzu.edu.cn