摘要
利用 SDS单向电泳对黄瓜 ( Cucumissativus L .)的根、茎、叶、花萼、花冠、雄蕊、花柱和子房的可溶性蛋白进行了分析和比较。检测到花冠中的 2 3.5 k D和 33.0 k D,雄蕊中的 1 8.8k D、2 8.5 k D、31 .0 k D、37.0 k D和 39.0 k D,花柱中的 4 5 .0 k D及子房中的 32 .5 k D蛋白 ,分别为各自器官中的器官特异蛋白质。对花冠、雄蕊、花柱和子房的可溶性蛋白的 IEF- SDS双向电泳分析也确定了相应于 SDS单向电泳上特异蛋白带的蛋白质斑点。而且相应于 SDS单向电泳上的一条带 ,在 IEF- SDS双向电泳上可能是一个以上的分子量相同而等电点不同的几个蛋白质斑点。各种器官的蛋白质含量以雄蕊为最高、花萼为最低。
Soluble proteins extracted from various organs of cucumber (Cucumis sativus L.) were analyzed using the technique of SDS one dimensional gel electrophoresis.The majority of the proteins were expressed in more than one organ,while those specific to only one organ were comparatively rare.Only two of all the proteins analized appeared to be truely organ specific.A 40.0 kD protein and a 30.8 kD protein were observed only in extracts of reproductive organs stamen and ovary.Some proteins were specific only to certain reproductive organ.They are 35.0 kD and 34.0 kD in sepal,18.8 kD,28.5 kD,31.0 kD,37.0 kD and 39.0 kD in stamen,45.0 kD in style with stigma and 32.5 kD in ovary.IEF SDS two dimensional electrophoresis showed that many of the one dimensional gel bands represented several proteins dots in the 2 D and that some of them were unique to an organ type of the flower.Protein analysis of the various organs showed that stamens contained the greatest amount and petals the least amount of protein.
基金
国家自然科学基金
关键词
黄瓜
器官特异性蛋白
Cucumber
SDS one dimensional electrophoresis
IEF SDS two dimensional electrophoresis
Organ specific protein
