摘要
用灭活O型口蹄疫病毒为抗原免疫BALB/c小鼠4次后,取致敏的脾B淋巴细胞和SP2/0小鼠骨髓瘤细胞进行融合,在HAT选择培养基中培养2周,经间接ELISA法筛选,最终获得了3株抗O型FMDV的阳性杂交瘤细胞株,命名为2F1、2G7和6H4。血清学试验证明该McAbs能与FMDV抗原结合,具有高度特异性。
To obtain monoclonal antibodies（McAbs） against foot-and-mouth disease virus（FMDV） O type, BALB/c mice were immunized with attenuated FMDV O type, and then lymphocytes from the splenic tissues of the mice were fused with SP2/0 myeloma cell. By limiting dilution,three monoclonal antibodies（McAbs） against FMDV named 2F1,6H4 and 2G7 were screened by the indirect ELISA. These McAbs could specifically recognize FMDV O type antigens in a serological test.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2007年第3期227-231,共5页
Chinese Veterinary Science
基金
国家高技术研究发展计划(863)项目(2003AA241110)
关键词
口蹄疫病毒
单克隆抗体
生物学特性
foot-and-mouth disease virus
monoclonal antibodies
biological characteristic
作者简介
林彤（1977-），男，四川中江人，硕士生，E-mail：1tth1@163．com。
常惠芸为通讯作者，Tel：0931-8342052，E-mail：changhuiyun@126．com
