摘要
以转化型人参皂苷的8个组分,按12.50和6.25μg·mL-1的剂量,以培养抗禽流感(AIV)H9亚型血凝素特异性单克隆抗体6E6株杂交瘤细胞和抗新城疫病毒(NDV)血凝素与神经氨酸酶单克隆抗体10B11株杂交瘤细胞,收集细胞培养上清,用血凝抑制试验(HI)和间接免疫荧光试验(IFA)检测单抗水平,探讨转化型人参皂苷对单克隆抗体生成的影响。结果表明;转化型人参皂苷3、4、7号组具有促进6E6单抗和10B11单抗生成的作用,其HI效价为26-27,与对照组(25)相比差异显著;IFA效价为104-105,与对照组(102)相比差异极显著。
Bioactivity of eight different transformed ginsenosides(TG) on hybridoma cells to producing immunoclonal antibodies was investigated. The 6E6hybridoma cell line producing specific to heamagglutinin of subtype H9avian influenza virus (AIV) and the 10B11 hybridoma cell line producing specific to heamagglutinin of Newcastle disease virus (NDV) and neuraminidase were cultured with the eight different transformed ginsenosides at the dose of 12.5 and 6.25μg·mL-1. The cultural supernatants were collected respectively and analysed by heamagglutination inhibition (HI) and indirect immunofluorescence assay(IFA). The results showed that TG-3, TG-4 and TG-7 could enhance the production of monoclonal antibodies in 6E6and 10B11celllines. The HI titers of the groups treated with TG-3, TG-4 and TG-7 was up to 26-27, which was significantly different from 25 of the control group, and the IFA titers 104-105 of groups exposed to TG-3, TG-4 or TG-7 was significantly differenl from 102 of the control group.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2005年第1期16-18,共3页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
江苏省动物预防医学重点实验室开放课题(K2077)扬州大学科研基金资助项目(10011143)
关键词
转化型人参皂苷
单克隆抗体
禽流感病毒
新城疫病毒
transformed ginsenoside
monocolnal antibodies
avian influenza virus
Newcastle disease virus
