摘要
目的:探讨大鼠肝硬化模型形成过程中门静脉压力及血流动力学的变化,建立有效的血流动力学特征研究模型.方法:选用雄性健康SD大鼠80只,随机分为4组,1组作为空白对照,其余3组建立大鼠肝硬化模型,分别于模型形成过程中第2,5,10wk观察门静脉压力及血流动力学变化特点,并与前一组为对照进行比较.结果:试验组在注射四氯化碳(CCl4)2wk后门静脉压力明显升高为(10.23±0.36)mm-Hg(1mmHg=0.133kPa),并在10wk内持续升高至(16.73±1.46)mmHg,各组与其对照组相比有统计学意义(P<0.01);门静脉内径、血流速度、血流量2wk后即明显升高,分别为(0.185±0.015)cm,(13.30±0.96)cm/s,(12.40±2.95)mL/min,与对照组相比差异有统计学意义(P<0.01),第5wk流速(12.22±1.18cm/s)与相应对照组比较有差异外,其余各项与相应对照相比差异均无统计学意义;充血分数注射后第2,5wk与相应对照比较差异均有统计学意义(P<0.01或P<0.05).结论:在制备大鼠肝硬化模型过程中,第2wk即可见明显血流动力学及压力改变,在第10wk可形成稳定的大鼠肝硬化门脉高压症的血流动力学研究模型.
AIM： To investigate the changes of hemodynamics and portal vein pressure in the progression of portal hypertension （PHT） in rats, so as to establish a study model of PHT hemodynamics. METHODS： Totally 80 healthy male SD rats were assigned to 4 groups （ 1 control group and 3 model groups）. Animal model of cirrhosis was made by subcutaneous injection with carbon tetrachloride （ CCl4 ）. The hemodynamic indexes and the portal vein pressure were assessed by ultrasonography at week 2, 5, 10 of modeling, and then the pathological changes of the livers were observed by serial slices with HE staining. RESULTS： During the formation of experimental cirrhosis, the hepatocytes of rats suffered from 4 processes including degeneration, necrosis, fibrosis and pseudolobular proliferation. The inner diameter, the hlood flow velocity and volume of portal vein sigaificantly raised to （0. 185 ±0.015） cm, （13.30 ±0. 96） cm/s, （ 12.40 ±2. 95） mL/min, in 2 weeks after CCl4 injection （ P 〈 0. 01 ）. But in 5 and 10 weeks after injection, there were no significant difference from control group, except the blood flow （ 12.22 ± 1.18 ） cm/s in 5 weeks. Pressure of portal vein in 2, 5, 10 weeks and congest index of portal vein in 2, 5 weeks were significantly different from the corresponding control group （P〈0.01 or P 〈0.05）. CONCLUSION： During the progression of PHT, there are significant changes of hemodynamics and portal vein pressure since 2nd week after CCl4 injection, and we can get a stable hemodynamics study model in 10th week after injection.
出处
《第四军医大学学报》
北大核心
2007年第5期404-406,共3页
Journal of the Fourth Military Medical University
作者简介
李鹏超，硕士生（导师鲁建国）．Tel：（029）84778265Email：lipic@163．com
通讯作者：鲁建国．Tel：（029）84777332Email：tdsurg@fmmu．edu．cn
