摘要
目的观察银杏酮酯(EGb_(50))对大鼠坐骨神经损伤后神经中诱导型一氧化氮合酶(iNOS)表达的影响。方法 SD 大鼠156只,随机分成假手术组、损伤对照组与实验组,后两组切断右侧坐骨神经并缝合。实验组给予 EGb_(50) 200 mg·kg^(-1)·d^(-1)溶于1 ml 生理盐水中灌胃,损伤对照组给予生理盐水1 ml 灌胃,假手术组仅显露右侧坐骨神经,术后不作处理。分别于术后1、3、7、14、21及28 d 取吻合口远端的神经,采用免疫组织化学和实时荧光定量聚合酶链反应(PCR)方法检测损伤神经组织中 iNOS 的表达水平。结果实验组 iNOS 阳性染色吸光度均值(A)在术后3、7、14和21 d均明显低于对照组(均 P<0.05),术后1、3和7 d,实验组坐骨神经中 iNOS mRNA 的表达均低于对照组(P<0.01)。结论银杏酮酯可抑制大鼠坐骨神经损伤后神经纤维中 iNOS 的表达,其促进神经再生的作用机制可能与抑制 iNOS 的表达有关。
Objective To investigate the effect of extract of Ginkgo biloba leaves （EGb50） on the expression of inducible nitric oxide synthase （iNOS） after sciatic nerve injury. Methods 156 male SD rats were randomly divided into 3 groups： experiment group （n = 72）, undergoing section and anastomosis of sciatic nerve and then gastric petfusion of EGb50 200 mg · kg^-1 · d^-1 ; injury control group （ n = 72 ）, undergoing section and anastomosis of sciatic nerve and then gastric perfusion of normal saline daily; and sham operation group （n = 12）, undergoing sham operation and gastric perfusion of normal saline daily. The rats were killed at different time points ： 1, 3, 7, 14, 21, and 28 days after the operation. The sciatic nerve distant to the anastomotic sites with a length of 0.5 cm was cut out to undergo immunohostochemistry and RT-PCR to detect the protein and mRNA expression of iNOS. Results No obvious iNOS protein and mRNA expression was seen in the sham operation group. 3, 7, 14, and 21 days after operation the mean values of absorbance of iNOS in the sciatic nerve specimens of the experiment group were all significantly lower than those of the injury control group （P 〈0.05 ,P 〈0.01 ）. High mRNA expression of iNOS began to be shown in the sciatic nerve specimens since lday after the operation and remained at a high level till 7 days after and then began to decrease in the injury control group. The levels of mRNA expression of iNOS in the sciatic nerve specimens 1,3, and 7 days after the operation of the experiment group were all significantly lower than those of the injury control group （ all P 〈 0.01 ）, and there was no significant difference in the levels mRNA expression of iNOS 14, 21, and 28 days after the operation between the experiment group and injury control group （all P 〉 0. 05 ）. Conclusion The extract of Gingko biloba promotes the regeneration of nervous tissues, probably by inhibiting the expression of iNOS.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2007年第7期485-488,共4页
National Medical Journal of China
基金
国家"973"重点基础研究发展计划基金(2003CB515305)
上海市科学技术委员会科研计划项目(04dz19855)
作者简介
通讯作者：王欢，Email：lhd@swk.sbmu.edu.cn
