摘要
试验结果表明:茎尖表面消毒用5%次氯酸钠比0.1%二氯化汞效果好。茎尖取材的来源对茎尖培养过程中的成活率和脱毒率没有影响。切取的茎尖越小,脱毒率越高,成活率越低。高温预处理对提高茎尖脱毒率效果明显。合适的激素配方可以促进茎尖的分化。
The effect with 5% NaClO was better than with 0.1% HgCl2 to the surface of stem tip in sterilizing. There was no influence to survival and virus-free percent in the course of cultural process of stem tip from different origin. The smaller stem tip being cut, the higher virus-free percent,and the lower survival percent. There was obvious effect on enhancing virus-free percent of stem tip with high temperature pretreatment. Suitable hormone formulae can promote stem tip divisional.
出处
《山西农业科学》
2007年第1期39-41,共3页
Journal of Shanxi Agricultural Sciences
关键词
马铃薯
茎尖培养
脱毒率
Potato
Stem tip culture
Virus-free percent
作者简介
王秀英（1967-）．女，山西大同人．助理研究员，主要从事植物组织培养研究工作。
