摘要
目的建立用RP-HPLC法测定生物体内士的宁浓度的方法。方法色谱柱为DIKMA Diamonsil^TM C18（250mm×4．6mm，5μm），流动相为乙腈-0．01％三乙胺溶液（用磷酸调节pH值至2．6）（12，88），流速为1．0mL／min，检测波长为254nm。结果士的宁在0．75～18．0μg／mL的浓度范围内与峰面积呈良好的线性关系，在血液和肝脏中的回归方程分别为：Y=106751X-17366，r=0．9996和Y=118967X-25910，r=0．9996（n=7）。3种不同浓度的日内、日间RSD均小于10．0％。回收率在90％～110％，血液及肝脏中士的宁的定量下限分别为0．15μg／mL和0．45μg／g。绪论此方法准确、灵敏，能够用于测定生物体内士的宁的浓度。
Objective To establish a RP-HPLC method to determine the concentration of strychnine in biological materials. Methods DIKMA DiamonsilTM C18 column （250 mm× 4.6 mm, 5 μm） was used as an analytical column. The mobile phase consisted of 12 volume of acetonitrile, 88 volume of 0.01% triethylamine （adjusted to pH 2.6 with phosphoric acid）. The flow rate was 1.0 mL/min. The UV detection wavelength was set at 254 nm. Results Strychnine had a good linearity in the range of 0.75-1.8μg/mL. The minimum detection concentration in blood plasma and liver was 0.15 μg/mL and 0.45μg/g, respectively. The recoveries of three different concentrations ranged from 90% to 110%, and the RSD values of Intra-day and inter-day were all under 10.0%. Conclusion The method is quick, precise and simple. It can be used to determine the concentration of strychnine in biological materials.
出处
《河北医科大学学报》
CAS
2007年第1期28-30,共3页
Journal of Hebei Medical University
作者简介
王春英（1972-）．女．河北高阳人．河北医科大掌药学院讲师，医学硕士，从事中药质量控制与体内测定研究。
通讯作者
