摘要
目的:研究进展期胃癌中胸苷磷酸化酶(TP)表达与癌组织分化程度、淋巴结转移及肿瘤内微血管密度(MVD)的关系,以及与5-氟脲嘧啶化疗患者预后的关系。方法:对60例进展期胃癌标本(患者术后均经过常规的以5-FU为主的化疗)及30例正常胃粘膜组织进行免疫组化染色SP法测定TP的表达情况;通过CD 31免疫组化染色检测癌组织中MVD。结果:胃癌细胞的TP表达阳性率明显高于正常胃组织(P<0.05);TP阳性组中MVD明显高于TP阴性组(P<0.01);TP阳性组中局部淋巴结转移率高于TP阴性组(P<0.05);胃癌中高、中、低分化的癌组织之间的TP阳性率无明显差异(P>0.05)。结论:胃癌中TP的表达与胃癌的血管生成、淋巴结转移及胃癌患者的预后关系密切,与胃癌的分化程度无关。
Objective ： To study the relationship of the expression of thymidine phosphorylase （TP） with the degree of differentiation , lymph node metastasis , intratumoral microvessel density （MVD） and prognosis with post-operative adjuvant chemotherapy with 5-FU of advanced gastric carcinoma. Methods： The expression of TP in 60 cases with advanced gastric carcinoma （all underwent post-operative adjuvant chemotherapy with 5-FU） and 30 cases with normal gastric mucosa was measured by method of SP of immunohistochemistry. And intratumoral microvessel density （MVD） was immunohistochemically detected using the method of SP of anti-CD31 monoclonal antibody. Results： The level of TP of cancer ceils was significantly higher than that of normal gastric epithelial ceils （P〈0.05）. In addition, MVD of the TP-positive group was greatly more than that of TP-negative group （P〈0. 01）. The frequency of lymph node metastasis of TP-positive group was also significantly higher than that in TP- negative group （P〈0. 05）. There was no difference among the level of TP in high-differentiated, middledifferentiated and poor-differentiated gastric carcinoma （P〉0.05）. Conclusion： The expression of TP in advanced gastric carcinoma is closely correlated with angiogensis, lymph node metastasis and its prognosis ,but is irrelevant to its differentiation.
出处
《陕西医学杂志》
CAS
北大核心
2006年第12期1576-1578,共3页
Shaanxi Medical Journal
基金
延边大学自然科学基金(2003-15)
关键词
免疫组织化学·肿瘤侵润
胃肿瘤/病理生理学
胸苷磷酸化酶
Immunohistochemistry Neoplasm invasiveness Stomach neoplasms/ physiopathology Thymidine pnospnorylase
