摘要
目的:探讨不同组织微环境中明胶酶在异种移植瘤中的表达差异及其对移植瘤生物学行为的影响.方法:?应用原位杂交方法分别检测皮下移植瘤及腹腔移植瘤中MMP-2?mRNA,?MMP-9?mRNA的表达情况,?并应用常规HE染色观察不同移植瘤的生物学行为表现.结果:?人胃癌裸小鼠皮下异种移植瘤呈膨胀性生长,?侵袭性不明显;?腹腔内移植瘤呈侵袭性生长;?MMP-2及MMP-9?mRNA在皮下移植瘤细胞及间质表达阴性,?在腹腔移植瘤为阳性表达,?且表达呈部位特异性,?即癌巢边缘部位的癌细胞胞质和/或包膜阳性表达.结论:?相同来源的瘤组织在不同的微环境中,?生长方式明显不同,?组织环境对肿瘤侵袭表型有很大影响;?明胶酶的表达与肿瘤生长方式及侵袭性有密切联系.
AIM： To study the expression of gelatinase （matrix metalloproteinase, MMP） and its effect on the biological behaviors of human gastric carcinoma xenografts in different organic microenvironment.
METHODS： In situ hybridization was used to detect the mRNA expression of MMP-2 and MMP-9 in tumors transplanted subcutaneously and intraperitoneally. HE staining was conducted to investigate the biological behaviors of the transplanted tumors.
RESULTS： The subcutaneous xenografts of human gastric carcinoma in nude mice presented an expanding growth style with limited invasion. In contrast, the intraperitoneal xenografts displayed an invasive growth pattern accompanied by more fibrous stroma. The mRNA expression of MMP-2 and MMP-9 were negative in the cells and intercellular substance of subcutaneous xenografts, but positive in the cytoplasm from the verge and envelope of intraperitoneal xenografts.
CONCLUSION： The tissue environment has definitive effects on the tumor phenotypes, and the expression of gelatinase is closely related to the growth pattern and invasion of tumor cells.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第32期3136-3138,共3页
World Chinese Journal of Digestology
基金
黑龙江省自然科学基金资助项目
No.D2005-11
关键词
明胶酶
侵袭
原位杂交
肿瘤移植
Gelatinase
Tumor invasion
In situ hybridization
Neoplasm transplantation
作者简介
通讯作者：李玉莲．150081．黑龙江省哈尔滨市南岗区哈平路150号．哈尔滨医科大学附属肿瘤医院，liyulian1@yahoo．com.cn，电话：0451-86298115，传真：0451-86298116
