摘要
用光谱探针溴化乙锭对劳氏青莲与脱氧核糖核酸的作用机制进行了研究。通过对体系的荧光光谱、吸收光谱及共振光散射光谱等方面的研究,结果表明,在pH为7.4的Tris-HCl缓冲溶液中,劳氏青莲与脱氧核糖核酸之间存在着嵌插作用,其结合常数为1.0×105L.mol-1。
The interaction between thionine and deoxyribonucleic acid was studied with ethidium bromide probe in Tris HCl buff er solution at pH 7.4 by fluorescence spectra, electronic absorption spectra, and resonance light scattering spectra. From the ob tained results, it was found obviously that the planar structure of thionine can be intercalated into the stacked base pairs of DNA, which is the maior controlling factor. The binding constant was found to be 1.0×10^5L·mol^-1.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2006年第10期1895-1898,共4页
Spectroscopy and Spectral Analysis
基金
河南省自然科学基金项目(0511021200)资助
关键词
荧光光谱
吸收光谱
共振光散射光谱
劳氏青莲
溴化乙锭探针
DNA
Fluorescence spectra
Absorption spectra
Resonance light scattering spectra
Thionine
Ethidium bromide probe
DNA
作者简介
刘雪平，1974年生，平顶山学院化学系教员，硕士
通讯联系人
