摘要
目的：研究抗癌新药吡柔比星（ＴＨＰ）与ＤＮＡ的作用方式。方法：利用荧光光谱法和吸收光谱法，以ＴＨＰ为荧光探针研究测定ＤＮＡ。结果：ＴＨＰ与ＤＮＡ的结合常数为１６６×１０５Ｌ·ｍｏｌ－１，结合位点数为２６ｂａｓｅｐａｉｒｓ。此外，利用ＤＮＡ对ＴＨＰ的荧光猝灭作用研究了对ＤＮＡ的分析应用。ＤＮＡ的检出限为０９１μｇ·ｍＬ－１，线性范围为０９１～９０μｇ·ｍＬ－１和９０μｇ·ｍＬ－１～２６μｇ·ｍＬ－１。结论：ＴＨＰ的蒽醌环与ＤＮＡ的碱基对发生嵌入作用，而ＴＨＰ的含糖侧链与ＤＮＡ有小沟槽作用。
AIM: To study the characteristics of the interaction of pirarubicin (THP) with DNA. METHODS: Study and determination of DNA with THP as a fluorescence probe was presented by absorption and fluorescence spectra. RESULTS: The binding constant of the interaction between THP and DNA is 1 66×10 5 L·mol -1 , and the binding site number is about 2 6 base pairs. The limit of detection is 0 91 μg·mL -1 and the linear ranges are 0 91～9 0 μg·mL -1 and 9 0～26 μg·mL -1 respectively for the determination of DNA. CONCLUSION: It indicates that the anthracycline moiety of THP intercalates the stacked base pairs of DNA, which is the major controlling factor. In addition, the complex sugar structure of THP may be bound to the minor groove of DNA double helix.
出处
《药学学报》
CAS
CSCD
北大核心
1999年第8期608-612,共5页
Acta Pharmaceutica Sinica
基金
高校博士点基金
湖南省科委基金
关键词
荧光光谱法
抗癌药
吡柔比星
嵌入作用
DNA
fluorescence spectrum
untitumor drugs
pirarubicin
DNA
intercalation
