摘要
目的研究齐墩果酸(OA)对环磷酰胺所致大鼠肝细胞损伤的保护作用。方法分离大鼠肝细胞,进行原代培养。检测实验大鼠肝细胞上清液中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)和肝细胞MTT值。结果环磷酰胺可使肝细胞上清液ALT、AST及LDH活力升高,肝细胞MTT值减小,齐墩果酸能抑制上述变化。结论齐墩果酸可抗环磷酰胺所致肝细胞损伤。
Objective To observe the toxicity of eyelophosphamide （CP） on primary euhured hepatoeytes and the protective effects of Oleanolie acid（OA）. Methods Rat hepatoeytes were isolated for primary euhure and intraeellalar ALT, AST and LDH leakages were detected in supernatant and MTT assay was used to evaluate the toxicity of CP and the protective potent of OA. Results CP increased the leakages of ALT,AST and LDH from hepatocytes with decreased the MTT value of hepatoeytes. These changes were ameliorated partly by OA. Conclusion OA can protect hepatoeytes from CP induced damage.
出处
《医药导报》
CAS
2006年第11期1114-1116,共3页
Herald of Medicine
关键词
齐墩果酸
环磷酰胺
肝细胞损伤
原代培养
Oleaolie acid
Cyelophosphamide
Injury of liver cell
Primary culture
作者简介
宫新江（1982-），男，山东青州人，在读硕士，主要研究方向为药理学。
[通讯作者]邱银生（1964-），男，湖北武汉人，研究员，博士。电话：027-88849945，E-mail：Qiuyinsheng6405@sohu．com
