摘要
目的:观察重组人促红细胞生成素对大鼠脊髓损伤后脂质过氧化和超微结构改变的影响。方法:实验于2004-05/08在吉林大学第二医院中心实验室完成。选择健康成年SD大鼠27只,采用改良Allen’s法制作脊髓损伤模型。27只SD大鼠随机数字表法分为3组:治疗组(n=9):造模后立刻给予重组人促红细胞生成素腹腔内注射(5000U/kg)1次;生理盐水对照组(n=9):造模后立刻给予等量生理盐水腹腔内注射1次。正常组(n=9):不作任何处置。采用硫代巴比妥酸法检测脊髓损伤后2,24,48h丙二醛含量变化,使用透射电镜技术评估脊髓损伤后各时相点的超微结构评分。结果:纳入大鼠27只,均进入结果分析。①生理盐水对照组和治疗组伤区丙二醛含量随伤后时间的增加呈不断升高的趋势,生理盐水对照组伤后2,24,48h伤区丙二醛含量较正常组明显增加,差异有显著性意义[分别为(92.45±6.22),(65.62±2.22)nmol/g;(112.56±6.68),(68.20±1.84)nmol/g;(142.38±7.24),(66.40±2.04)nmol/g,P<0.05]。治疗组伤后2,24,48h丙二醛含量较生理盐水对照组明显降低[分别为(68.54±5.88),(92.45±6.22)nmol/g;(75.24±6.28),(112.56±6.68)nmol/g,P<0.05;(88.34±8.52),(142.38±7.24)nmol/g,P<0.01]。②生理盐水对照组的超微结构评分随伤后时间的增加呈不断升高的趋势,而治疗组的超微结构评分随伤后时间的增加则变化不大,相对稳定。治疗组伤后2,24,48h的超微结构评分均较生理盐水对照组明显降低,差异有显著性意义[分别为(1.28±0.45),(3.84±0.25)分;(1.38±0.28),(4.24±0.38)分;(1.42±0.36),(4.98±0.52)分,P<0.05]。结论:重组人促红细胞生成素能够明显降低脊髓损伤后丙二醛含量,减轻脊髓损伤后的脂质过氧化,显著降低脊髓损伤后的超微结构评分,明显减轻脊髓损伤后的超微结构改变,有效地保护脊髓组织,具有明显的神经保护作用。
AIM： To observe the effects of recombinant human erythropoietin （rHuEPO） on the lipid peroxidation and uhrastructure after spinal cord injury （SCI） in rats.
METHODS： The experiment was carried out at the Central Laboratory in Second Hospital of Jilin University from May to August 2004. A total of 27 healthy adult SD rats were selected. By the modified Allen＇s method, the model of SC1 is established, 27 SD rats were randomly divided into three groups： the rats in the treatment group （n=9） were subjected to rHuEPO by intraperitoneal injection （5 000 U/kg） once after establishing models. The rats in the saline control group （n=9） were subjected to equal volume saline by intraperitoneal injection after establishing models once. Normal group （n=9）： no any intervention. The content of malondialdehyde after SCI were measured at hours 2, 24 and 48 with thiobarbituric acid method. The uhrastructure change of injured spinal cord tissue at every time point after spinal cord injury was observed with transmission electron microscopy.
RESULTS： Totally 27 included rats were involved in the result analysis. （1）The content of malondialdehyde of injured spinal cord increased with time shift in saline control group and treatment group. The content of malondialdehyde of injured spinal cord increased significantly at hours 2, 24 and 48 in the saline control group compared with normal group, and there was significant difference [（92.45±6.22）, （65.62±2.22） nmol/g; （112.56±6.68）, （68.20±1.84） nmol/g; （ 142.38±7.24）, （66.40±2.04） nmol/g, P 〈 0.05]. The content of malondialdehyde of injured spinal cord decreased significantly at hours 2, 24 and 48 in the treatment group compared with the saline group [（68.54±5.88）, （92.45±6.22） nmol/g; （75.24±6.28）, （112.56±6.68） nmol/g,P 〈 0.05 ; （88.34±8.52）, （ 142.38±7.24 ） nmol/g, P 〈 0.01]. （2）The uhrastructure in the saline control group increased gradually with the increase of time, while the uhrastructure score in the treatment group did not change with the increase of time, relatively stable. The uhrastructure score decreaged markedly at hours 2, 24 and 48 in the treatment group compared with the saline control group, and there was significant difference [（1.28±0.45）, （3.84±0.25） points; （1.38±0.28）, （4.24±0.38） points; （ 1.42±0.36）, （4.98±0.52） points,P 〈 0.05].
CONCLUSION： The rHuEPO can decrease remarkably the content of malondialdehyde after spinal cord injury, relieve the lipid peroxidation after spinal cord injury, decrease significantly ultrastructure score of injured spinal cord, release remarkably uhrastructure change of injured spinal cord, protect effectively spinal cord tissue, and have distinctly neuroprotective effect.
出处
《中国临床康复》
CSCD
北大核心
2006年第44期78-80,共3页
Chinese Journal of Clinical Rehabilitation
作者简介
任宪盛，男，1974年生，辽宁省大连市人，汉族，吉林大学第一医院在读博士，主治医师，主要从事脊柱脊髓损伤及疾病的研究。
通讯作者：杨有庚，教授，硕士，博士生导师，吉林大学第二医院骨科，吉林省长春市130041
