摘要
目的:建立评价苦参质量优劣的指纹图谱分析方法。方法:利用RP-HPLC-DAD法,采用梯度洗脱模式测定了24批苦参样品。样品采用两种溶剂分别提取并分别分析测定,其黄酮类成分测定色谱条件:Hypersil ODS_2(200mm×4.6mm,5μm);柱温35℃;流动相A-乙腈,流动相B-0.05%磷酸水溶液,梯度洗脱程序0min:A-B(6:94),42min:A-B(32:68),80min:A-B(70:30);流速1.0mL·min^(-1)。生物碱类成分测定色谱条件:Hypersil ODS_2(200mm×4.6mm,5μm);柱温35℃;流动相:甲醇-乙腈-水-磷酸(10:28.5:61.5:0.05,含0.8%SDS);流速0.8mL·min^(-1)。结果:确定了苦参黄酮类和生物碱类成分指纹图谱中的46个共有峰,根据聚类分析和相似度分析结果,将苦参药材分为3类。结论:本法重复性好,专属性强,为科学评价苦参药材质量提供了依据。
Objective ：To establish the RP - HPLC fingerprint analysis for the quality control of Sophora flavescens Ait. Methods：RP - HPLC - DAD method was applied in the chromatographic separation. The HPLC analysis for flavonoids was performed on a Hypersil ODS2 column（ 200 mm × 4. 6 mm ,5 （m） with the mixture of acetonitrile（A） and 0. 05% phosph ate acid solution（B） as mobile phase in gradient mode. The concentrations of solvent A were 6% ,32% and 70% at 0,42 and 80 min,respectively. The column temperature was setup at 35 ℃ and the flow - rate was 1.0 mL· min^-1. The HPLC analysis for alkloids was carried on the same column with a mobile phase of methanol - acetonitrile - water - phosphate acid（ 10： 28.5： 61.5： 0. 05,0. 8% SDS） in isocratic mode ,the flow rate being 0.8 mL min^- 1 and the the column temperature being the same as the conditions above. Results：In the finger-print ,46 common peaks in Sophoraflavescens Ait were confirmed, and the 24 batches of Sophoraflavescens Ait. were classified to be recommended, unrecommended and unqualified ones based on the results of cluster analysis and similarity analysis. Conclusion： The methods can be used scitentifically to evaluate the quality of Sophora flavescens Ait.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第9期1261-1265,共5页
Chinese Journal of Pharmaceutical Analysis
关键词
苦参
RP—HPLC
指纹图谱
Sophora flavescens Ait
RP - HPLC
chromatographic fingerprint
作者简介
通讯作者Tel：（024）23843711—3386；E—mailksbi@mail．s．y.ln.cn
