摘要
目的了解杭州地区金黄色葡萄球菌(简称金葡菌)对苯唑西林的耐药情况,探索PCR法检测耐药性相关的mecA基因的敏感性和特异性。方法用金葡菌乳胶凝集试验和Vitek系统GPI卡鉴定金葡菌,用纸片扩散法和E-test法进行药敏试验,并对所有菌株均采用PCR技术检测与耐药性相关的mecA基因。结果Kirby-Bauer法和E-test法检测金黄色葡萄球菌对苯唑西林的敏感性,133株中敏感株分别为113株(85·0%)和115株(86·5%),中介株分别为2株(1·5%)和4株(3·0%),耐药率(株)分别是18株(13·5%)和14株(10·5%);所有菌株均对万古霉素敏感。mecA基因的PCR扩增结果显示:所有的苯唑西林敏感株mecA基因均阴性,中介株mecA基因阳性1株,而所有耐药株均阳性,标准株ATCC25923则为阴性。杭州地区金葡菌对苯唑西林的耐药率为10·5%,以E-test法为金标准,纸片扩散法和PCR法的耐药菌检出的敏感性为100%,而特异性分别为96·6%和98·3%。结论金葡菌对苯唑西林的耐药率不高,而mecA-PCR技术可以作为快速检测耐苯唑西林金葡菌的有效方法。
Objective To study the antimicrobial agents resistance of staphylococcus aureus in Hangzhou area and the sensitivity and specificity of the mecA gene PCR assay. Methods A total of 133 staphylococci strains were isolated from the children in Hangzhou, then Disc Diffusion and staphylococcal mecA gene PCR assay for detecting MRSA were compared with E-test method. Results Of all 133 strains performed with both Kirby-Bauer and E-test methods in the susceptibility test,85.0% and 86.5% of the isolates were susceptible to oxacillin respectively. All of the isolates were susceptible to vancomycin with E-test MICs ≤4. 0 ug/ml,and 91.7% of them had the zone diameters not more than 15mm. The sensitivity of Disc Diffusion and mecA gene PCR assay for detecting MRSA were 100% respectively, the Disc Diffusion specificity was 96.6% ,and the mecA gene PCR assay＇s specificity was up to 98. 3%. Conclusion Rate of oxacillin- resistance staphylococcus aureus is low in the study, and mecA gene PCR assay is significant in rapid detection of MRSA.
出处
《中国实用儿科杂志》
CSCD
北大核心
2006年第9期673-676,共4页
Chinese Journal of Practical Pediatrics
作者简介
E—mail：ate215@126．com
