摘要
目的:观察重组人粒细胞集落刺激因子(recombinant human granulocyte colony stimulating factor;rhG-CSF)对D-氨基半乳糖(D-galactosamine,D-GalN)加脂多糖(lipopolysaccharide,LPS)联合诱导的小鼠急性肝衰竭的保护作用,并探讨其机制。方法:D-GalN/LPS腹腔联合注射制造小鼠急性肝衰竭模型,治疗组小鼠于造模前4h,2h及造模同时予以G-CSF300μg/kg腹腔注射,观察小鼠24h存活率。造模后6h每组随机选择5只小鼠处死,观察肝组织损伤情况,用半定量逆转录-聚合酶链反应和TanonGis3.73软件分析各组小鼠肝组织中肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)、白介素-6(IL-6)和白介素-10(IL-10)的表达情况。结果:治疗组小鼠24h存活率明显高于对照组(P<0.01),造模后6h治疗组小鼠肝组织损伤明显减轻(P<0.05),肝组织中TNF-α和IFN-γ的mRNA表达水平明显低于对照组(P<0.01),IL-6和IL-10的mRNA表达水平明显高于对照组(P<0.01)。结论:G-CSF对D-GalN/LPS所致的小鼠急性肝衰竭具有保护作用。
Objective To evaluate the protective effect of recombinant human granulocyte colo-ny stimulating factor （rhG-CSF） on acute hepatic failure induced by galactosamine （D-GaIN） and lipopolysaccharide （LPS） in mice, and to explore its mechanism. Methods The mice were intraperitoneally administered D-GaIN （800 mg/kg） and LPS （10μg/kg） , and then were intraperitoneally injected either saline （the control group ）or rhG-CSF at 300 μg/kg body weight （the therapy group ） at 4 h, 2 h and 0 h before the D-GaIN/ LPS injection. The survival rate of the mice was estimated at 24h after the D-GaIN/ LPS injection. The degree of hepatic injury was evaluated at 6 h after the D-GaIN/ LPS injection, and the levels of TNF-α, IFN-γ, IL-6 and IL-IO mRNA were simultaneously measured by semiquantitative RT-PCR. Results The survival rate of the therapy group was significantly higher than that of the control group （68.4% vs 20% , P 〈 0. 01 ）. As compared with the control group, the degree of liver injury in the therapy group significantly decreased （ P 〈 0.05 ） , and the levels of TNF-α and IFN-γ mRNA in the hepatic tissue also reduced remarkably （P 〈0.01, respectively）, while the levels of IL-6 and IL-IO mRNA increased （P 〈 0.01 , respectively） at 6 h after the D-GalN/ LPS injection. Conclusion G-CSF can protect the mice from acute hepatic failure induced by D-GalN/LPS.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2006年第4期543-547,共5页
Journal of Central South University ：Medical Science
关键词
急性肝衰竭
粒胞集落刺激因子
脂多糖
细胞因子
acute hepatic failure
granulocyte colony-stimulating factor
lipopolysaccharide
cytokine
作者简介
徐旭雯（1978-），女，江西上饶人，博士研究生，主要从事病毒性肝炎的研究。
通讯作者，E-mail：DMT2008@yahoo．com．cn
