摘要
目的本研究制备载硫酸长春新碱(vincristinesulfate,VCR)微球的胶原-壳聚糖缓释药膜。并考察加入壳聚糖对药膜性质的影响。选定适当的胶原壳聚糖比例制备药膜。方法采用W/O/O溶剂挥发法制备VCR的聚乳酸-羟基乙酸(poly(lactic-co-glycolicacid),PLGA)微球,并对微球性质表征,采用二次冻干法制备载VCR微球的胶原-壳聚糖药膜,对药膜的表面形态、降解性质、热力学性质及释放性质进行表征,并与释放2周后的药膜进行比较。采用高效液相法分析药物含量。结果VCR制成PLGA微球后再制备成药膜,可达到双重缓释的作用,明显减少药物突释,并延缓药物释放。添加了壳聚糖的药膜降解速度明显小于单纯的胶原药膜。在体外释放实验中,微球突释为(27.2±1.2)%,而胶原药膜的突释为(20.4±1.9)%,胶原与壳聚糖比例为9∶1、4∶1、3∶2的药膜突释分别为(20.2±2.1)%、(18.0±1.1)%和(16.3±1.8)%。结论胶原壳聚糖载VCR的缓释药膜能不同程度减少药物的突释,使药物释放更加平稳缓慢,优于单纯的胶原药膜。
Objective The objective of this study is to investigate a collagen-chitosan complex film with embedded vincristine sulfate （VCR） microspheres （MSs）. Methods A water-in-oil-in-oil double-emulsion/solvent evaporation method was used to prepare VCR-loaded PLGA microspheres. The microspheres were further mixed with collagen and/or chitosan swelling solution, followed by lyophilizing. The films were cross-linked by using 0.3% glutaraldehyde. Encapsulation efficiency, release kinetics of VCR microspheres, release kinetics as well as in vitro degradation of the film were evaluated. Results VCR was released from the film in a prolonged period and the initial burst release was less significant. The initial release was （27.2±1.2）% for the naked MSs. After the MSs were embedded in the films of collogan and collagen-chitosan （9：1,4：1,3：2, v/v）, the burst release decreased to （20.4±1.9）%,（20.2±2.1）%,（18.0±1.1）%,（16.3±1.8）%, respectively. In the degradation experiment, the film containing chitosan degraded more slowly than that of without chitosan. Conclusion The collagen-chitosan complex film could achieve the release kinectics of a relatively constant release.
出处
《国际生物医学工程杂志》
CAS
2006年第4期193-196,共4页
International Journal of Biomedical Engineering
基金
国家863计划课题资助项目(2002AA326040)
关键词
硫酸长春新碱
聚乳酸-羟基乙酸
微球
胶原
壳聚糖
vincristine sulfate
poly（lactic-co-glycolic acid）
microspheres
collagen
chitosan
作者简介
陈红丽（1978-），女，河南省人，硕士研究生
通讯作者：张其清，300192（E-mail：zhangqiq@xmu．edu．cn）
