摘要
目的：建立血中华法林浓度的毛细管胶束电动色谱分析方法，并对其进行评价。方法：用二氯甲烷提取血清中华法林，５－乙基－５－Ｐ－甲苯基巴比妥酸为内标，操作电压１５ｋＶ，２１０ｎｍ波长检测。电泳缓冲液由２５ｍｍｏｌ／Ｌ硼酸盐，２５ｍｍｏｌ／Ｌ十二烷基硫酸钠，１０ｍｍｏｌ／Ｌ氯化钠（ｐＨ９．０）组成。结果：日内ＲＳＤ１．４％～６．２％，日间ＲＳＤ１．４％～４．７％，在０～１０μｇ／ｍｌ范围内呈线性，最低血清华法林检测浓度为０．０３μｇ／ｍｌ。测定结果与反相高效液相色谱法进行了比较，两种方法有很好相关性，ｒ＝０．９９６７（Ｐ＞０．１）。结论：毛细管胶束电动色谱法精密度、准确度高，专一性强，是一种较理想的血药浓度检测方法。
OBJECTIVE:Toevaluateaselectiveprocedureforthedeterminationofwarfarininserumbymicel-larelectrokineticcapilarychromatography.METHODS:Extractionofthedrugwithdichlormethaneand5-ethyl-5-p-tolylbarbituricacidwasusedasinternalstandard,appliedvoltage15kV,detectedat210nmwavelength.Therunbufercontained25mmol/Lborate,25mmol/Lsodiumdodecylsulphate,and10mmol/Lsodiumchloride(pH9.0).RESULTS:Theprecisionofthemethod,expressedasrelativestandarddeviation,within-dayrangedfrom1.4%to6.2%,anddaytodayfrom1.4%to4.7%.Themethodwaslinearfrom0to10μg/mlandthelimitofdetectionwas0.03μg/ml.ComparedwiththoseobtainedbyHPLC,theconcentrationofwarfarinobtainedbythetwometh-odshadgoodlinearrelationshipwithaslopeof0.9975andacorrelationcofficientof0.9969(P>0.1).CONCLU-SION:Themethodshowedagoodprecisionandaccuracy,andissuitablefordeterminationofwarfarininserum.
出处
《中国药学杂志》
CAS
CSCD
北大核心
1996年第6期358-360,共3页
Chinese Pharmaceutical Journal
关键词
华法林
高效液相色谱
MECC
抗凝血药
warfarin,micelarelectrokineticcapilarychromatography,highperformanceliquidchromatography
