摘要
目的:用非抑制型电导检测离子排斥色谱法分离测定大青叶中有机酸的含量。方法:以2.2 mmol/L对甲苯磺酸为流动相,流量为0.6 mg/mL,柱温40℃。结果:大青叶中顺丁烯二酸、柠檬酸、DL-苹果酸、琥珀酸和反丁烯二酸5种有机酸的含量分别为8.04×103、3.32×104、3.00×104、506.42、389.84 mg/kg。结论:该方法快速、简便。
Objective： To establish a non - suppressed ion exclusion chromatographic method with conductivity detector for the separation and determination of organic acids in indigowoad leaf. Methods ： The aqueous solution of 2.2mmoL/L p - toluenesulfonic acid was used as mobile phase with the flow rate of 0.6 mg/mL. The temperature of the column was 40QC. Results ： The content of maleic acid, citric acid, malic acid, succinic acid and fumaric acid were 8.04×10^3 mg/kg,3.32×10^4 mg/kg, 3.00×10^4 mg/kg,506.42 mg/kg and 389.84 mg/kg,respectively. Conclusion： The method was rapid and convenient.
出处
《辽宁中医药大学学报》
CAS
2006年第4期120-121,共2页
Journal of Liaoning University of Traditional Chinese Medicine
关键词
离子排斥色谱法
有机酸
大青叶
ion exclusion chromatography
organic acids
indigowoad leaf
作者简介
张立（1977-），女，河北定州人，硕士研究生，从事色谱分离研究。
