摘要
目的:研究曲安奈德在增生性视网膜病变中不同时期给药的治疗效果及其与血管内皮生长因子的相互作用机制。方法:建立氧诱导的血管增生性视网膜病变幼鼠模型。预先治疗组于进氧箱前(出生7d),晚治疗组于出氧箱后(出生12d)幼鼠玻璃体腔内分别注射曲安奈德1μL(0.04mg),对侧眼注射相同体积的平衡盐溶液(balancedsaltsolution,BSS)作为对照。应用荧光素灌注荧光血管造影法,了解视网膜血管改变;取幼鼠眼球作普通病理切片及免疫组化检测,分别检测视网膜新生血管芽内皮细胞数目及VEGF的表达。结果:预先治疗组视网膜新生血管芽内皮细胞数目及VEGF的表达(8.00±1.21)显著少于给氧对照组(24.89±1.53)和晚治疗组(19.57±1.16);预先治疗组和晚治疗组分别与它们的对照眼及给氧对照组相比均有显著意义;预先治疗组和晚治疗组相比有显著意义(P<0.01)。结论:病变早期应用曲安奈德治疗效果明显优于病变晚期治疗。在氧诱导的血管增生性视网膜病变模型中,曲安奈德可以抑制视网膜新生血管形成和VEGF的表达。
AIH： To study the therapeutic effect of the triamcinolone acetonide （TA） on the different stages of proliferative retinopathy and its interaction with vascular endothelial growth factor （VEGF）. METHODS： Hodels of vascular proliferation retinopathy were established. Triamcinolone acetonide 1μL（0.04mg） was administered by intravitreal injection on day 7 in the pretreatment group （received treatment before oxygen exposure）. The late-treatment group （after oxygen exposure） received TA at the same dose on day 12. The same volume of BSS was injected into the other eye of the mice both in these two groups as controls. Retinal neovascularization was examined by fluorescein dextran angiography. Both eyeballs of newborn mice were enucleated for performing pathological sections and were studied by immunohistochemical method in order to count the nuclei of proliferative retinal vessels and to investigate the expression of VEGF in the retina.
RESULTS： Pretreatment reduced extraretinal vascularization, when assessed by quantification of neovascular nuclei, to a mean± SEH of 8.00＋1.21, significantly less than that in the untreated oxygen-exposed group （24.89 ± 1.53; P〈0.01） and late-treatment group （19.57± 1.16; P〈0.01）. Significant differences, indicated by quantification of neovascular nuclei and the expression of VEGF in the retina, was observed in the pretreatment （P〈0.01） and late-treatment （P〈0.01） groups when compared with the control group and the untreated oxygen-exposed group. Significant difference was observed in the pretreatment when compared with the late-treatment group.
CONCLUSIONS： The effect of pretreatment is superior to that of late-treatment. TA significantly inhibits retinal neovas- cularization and the expression of VEGF in a mouse model of retinal neovascularization.
出处
《国际眼科杂志》
CAS
2006年第3期587-590,共4页
International Eye Science
关键词
视网膜新生血管化
血管内皮生长因子
曲安奈德
玻璃体视网膜病
增生性
retinal neovascularization
vascular endothe-lial growth factor
triamcinolone acetonide
vitreoreUnopathy
proliferative
作者简介
许建华，女，副主任医师，博士研究生，研究方向：眼底疾病的研究。
通讯作者：刘哲丽，导师，zheli_liu@163.com
