摘要
为了获取足够的DNA片段或得到基因的表达产物,常需要构建基因串联体,将目的基因按头尾相连随机串联起来,克隆入克隆载体以制备需要的目的基因片段或克隆入表达载体进行高效表达。串联的策略包括定向串联法、PCR扩增串联法、化学拼接法、同尾酶法等,不同方法构建的串联体基因表达模式也有一定差异。
The great attempts to clone a gene in the form of muhimers were motivated either by the need to produce copious amounts of the particular DNA fragments or by the desire to obtain a large supply of the gene product of interest. The arrangement of the gene muhimeric copies is in identical tandem orientation, this head-to-tail arrangement of gene muhimers could be constructed by the strategies of tandem repeats, PCR amplification, chemical concatenation and isocaudarners. The expression mode of the gene muhimers may be different based on variable construction strategy.
出处
《医学研究生学报》
CAS
2006年第6期557-560,共4页
Journal of Medical Postgraduates
基金
国家自然科学基金资助项目(批准号:30171119
30571666)
军队"十五"医药卫生重点课题资助项目(批准号:01Z070)
关键词
串联体
构建
表达
Multimer
Construction
Expression
作者简介
饶贤才（1967-），男，浙江江山人，副教授，医学博士，从事抗微生物感染方面研究。
